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12 polyacrylamide gel using method

  • Polyacrylamide gel method: synthesis and property of

    2010-9-24 · Similarly, polyacrylamide gel method takes advantage of the network structures of gels and, more significantly, the polymerization reaction forming gel. During this process, the free-radical crosslinking copolymerization of acrylamide (AM) and N,N′ -Methylenebis(acrylamide) (MBAM) monomers happens in solutions containing cations and anions.

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  • The principle and method of polyacrylamide gel ...

    2020-11-23 · The gel-drying method described here normally takes about 24–36 h for complete drying, depending on the concentration and thickness of the polyacrylamide gels. For example, a 1.5-mm thick 7.5% polyacrylamide gel can dry in about 24 h, whereas gels of the same thickness with a 4–20% polyacrylamide gradient may take up to 36 h to dry completely.

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  • A Novel Method for Polyacrylamide Gel Preparation

    2021-3-24 · A modified polyacrylamide gel method has been used to synthesize the CeO2 nanoparticles by using cerium sulfate as metal source. The method makes full use of exothermic reaction of cerium sulfate in the precursor solution to polymerize acrylamide and methylene diacrylamide to obtain gel.

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  • A simple method of drying polyacrylamide slab gels

    2018-10-20 · Electrophoresis through agarose or polyacrylamide gels is a standard method used to separate, identify and purify biopolymers, since both these gels are porous in nature. Polyacrylamide gels are chemically cross-linked gels formed by the polymerization of acrylamide with a cross-linking agent, usually N,N’-methylenebisacrylamide.

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  • Modified polyacrylamide gel synthesis of CeO 2 ...

    2013-8-1 · A polyacrylamide gel method was used to prepare nanosized europium doped forsterite (Mg 2 SiO 4:Eu 3+). An amorphous nanopowder was synthesized. The single-phase forsterite could be formed at a relatively low temperature of about 700 °C without unreacted phases. Average particle size of the powders was about 50 nm. Few agglomerates were observed in the powders.

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    A more recent Protocol discussing this method is available Protocol Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method Michael R. Green and

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  • Polyacrylamide gel synthesis and sintering of

    2020-5-23 · Barium hexaferrites (BaFe 12 O 19) nanoparticles are prepared using γ-ray irradiation assisted polyacrylamide gel method (RIAPGM). The experiment parameters of as-prepared samples are investigated in detail, such as polymerization initiation mode and annealing temperature.

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  • Isolation of DNA Fragments from Polyacrylamide Gels

    (Received March 5/April 12, 1974) A simple method is described for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film. The gel is dehydrated in dimethyl sulphoxide, soaked in a solution of 2,5-diphenyloxazole (PPO) in dimethylsulphoxide, dried and …

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  • The principle and method of polyacrylamide gel ...

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-3-5 · objectives can be met using protein electrophoresis (Zewart and Harrington 1993). Polyacrylamide Gel Electrophoresis (PAGE) When electrophoresis is performed in acrylamide or agarose gels, the gel serves as a size-selective sieve during separation. As proteins move through a gel in response to an electric field, the gel’s pore structure

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  • A method for sensitive staining of DNA in

    Results: A fast and sensitive visible dye-based staining method for DNA in polyacrylamide gels using basic fuchsin (BF) is described. As low as 10-20 pg of DNA can be visualized within 10 min; the sensitivity is fourfold more sensitive than that of SYBR® Gold stain, the most sensitive commercial fluorescent probe, but similar to silver ...

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  • Scientific Protocols - A method for labeling ...

    2011-3-24 · Using this novel method of polyacrylamide gel labeling will make life easier for scientists dealing with many protein samples on a day to day basis and will enhance the accuracy of results by eliminating any ambiguity in recognition of the correct gel used to analyse a set of protein samples.

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  • Simplified method for the diameter sizing of serum

    1. Clin Chem Lab Med. 2004;42(9):1009-12. Simplified method for the diameter sizing of serum low-density lipoprotein using polyacrylamide gradient gel electrophoresis. Tsukamoto H(1), Takei I, …

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  • A Novel Method for Polyacrylamide Gel Preparation

    ORIGINAL RESEARCH published: 24 February 2021 doi: 10.3389/fmats.2021.637278 A Novel Method for Polyacrylamide Gel Preparation Using N-hydroxysuccinimide-acrylamide Ester to Study Cell-Extracellular Matrix Mechanical Interactions Jun Kumai 1, Satoru Sasagawa 1, Masanobu Horie 2 and Yoshihiro Yui 1* 1 Research Institute, Nozaki Tokushukai Hospital, Osaka, Japan, 2Radioisotope …

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  • Isolation of DNA Fragments from Polyacrylamide

    Protocol Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method . Joseph Sambrook and; David W. Russell; Cold Spring Harb Protoc; 2006; doi: 10.1101/pdb.prot2936

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  • Nondenaturing Polyacrylamide Gel Electrophoresis

    1994-1-1 · Steps 1 . T h e ' s a n d w i c h ' o f plates and spacers can be sealed by any standard method, such as sealing the edges with 1 % agarose or enclosing it in a casting chamber. Thirty-three milliliters o f gel solution is sufficient to cast four, 8 X 1 0 cm minigels, 0 . 7 5 m m thick, using the Hoefer S E - 2 7 5 casting c h a m b e r .

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  • Silver staining DNA in polyacrylamide gels | Nature

    2007-10-25 · This protocol describes a simple silver staining method used to visualize DNA fragments and other organic molecules with unsurpassed detail following traditional polyacrylamide gel electrophoresis ...

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  • Synthesis of SrAl 2 O 4 :Sm nanopowder using

    2016-6-8 · SrAl2O4:Sm nanopowders were synthesized using two different methods; polyacrylamide gel and reverse microemulsion with two different surfactants (CTAB and SDS). Morphology, thermal behavior, structural and photoluminescence properties were determined and compared using scanning electron microscopy, transmission electron microscopy, thermogravimetric and differential thermal …

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  • The principle and method of polyacrylamide gel ...

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

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  • Purificationof DNA using nondenaturing

    2016-8-9 · Purificationof DNA using nondenaturing polyacrylamide gel electrophoresis. JackD. Pollard, Jr. ... If the sample is to be UV shadowed (UNIT 2.12), then 2 mg of DNAwill be required per band in a 2 cm x 2 cm x 1.6 mm well. ... These two qualities make polyacrylamide gelsthe preferred method for purifying significant quantities of small fragments.

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  • Polyacrylamide Gel Electrophoresis - CSH Protocols

    Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of DNA whose lengths differ by as little as 0.1% (i.e., 1 bp in 1000 bp). (2) They can accommodate much larger quantities of DNA than agarose gels. Up to 10 µg of DNA can be applied to a single ...

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  • Affinity-Trap Polyacrylamide Gel Electrophoresis: A

    2009-12-28 · A method for the affinity capture of specific proteins from a complex mixture using a polyacrylamide gel technique is described. The approach is based on the orthogonal electro-transfer of proteins separated by ordinary polyacrylamide gel electrophoresis (PAGE) to a ligand-coupled polyacrylamide gel (Li-PAG), which is placed under the PAGE gel. Upon electro-transfer, the proteins …

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  • Markers using a Non-denaturing Polyacrylamide Gel A

    2018-4-20 · In addition, polyacrylamide gel can be divided into denaturing and non-denaturing polyacrylamide gels, and both can be used for the detection of SSR markers using the silver staining method. The effect and resolution of which do not significantly differ, but non-denaturing polyacrylamide gels are easier to process and take less time16.

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2016-10-14 · objectives can be met using protein electrophoresis (Zewart and Harrington 1993). Polyacrylamide Gel Electrophoresis (PAGE) When electrophoresis is performed in acrylamide or agarose gels, the gel serves as a size-selective sieve during separation. As proteins move through a gel in response to an electric field, the gel’s pore structure

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  • Denaturing Polyacrylamide Gel Electrophoresis

    2019-2-5 · Place gel sandwich in electrophoresis apparatus and clamp plates to support. Sweep out any air bubbles at bottom of gel by squirting buffer between plates using syringe with a bent 20-G needle. 9. Pour 1× TBE buffer into top reservoir to ∼3 cm above top of gel. Rinse top of gel with 1× TBE buffer using a Pasteur pipet or Beral thin stem. 10.

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  • Scientific Protocols - A method for labeling ...

    2011-3-24 · Using this novel method of polyacrylamide gel labeling will make life easier for scientists dealing with many protein samples on a day to day basis and will enhance the accuracy of results by eliminating any ambiguity in recognition of the correct gel used to analyse a set of protein samples.

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  • The method of dehydration and purification of ...

    (57) Abstract: The method of dehydration and purification gel of polyacrylamide includes freezing, grinding and processing solvent. While remaining solvent from the dehydrated and purified particles are removed by a stream of air or inert gas at a temperature not exceeding 48 o C. expanding the number of solvents used for the dehydration and purification of the gel from impurities.

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  • Fast reversible single‐step method for enhanced band ...

    (C) Analysis of the rate of reaction using polyacrylamide gels of varying percentages. Commonly used gel percentage formulations: 8, 10, 12, 14, and 16% were treated with absolute ethanol. The various gels : 8, 10, 12, 14, and 16%, turned opaque at the respective time points: 15, 20, 20, 25, and 30 min.

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  • Visible fluorescent detection of proteins in polyacrylamide ...

    2014-9-10 · 12% SDS–PAGE 1-mm thick gel as per the standard Laemmli method using the Protean II gel system from Bio-Rad Laboratories (Hercules, CA, USA) or the Atto Electrophoresis (AE-6450) Dual Mini Slab system (Atto Corp.). The low-molecular-weight standards from Bio-Rad Laboratories, contained phosphorylase b (97kDa,

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  • Purificationof DNA using nondenaturing

    2016-8-9 · Purificationof DNA using nondenaturing polyacrylamide gel electrophoresis. JackD. Pollard, Jr. ... If the sample is to be UV shadowed (UNIT 2.12), then 2 mg of DNAwill be required per band in a 2 cm x 2 cm x 1.6 mm well. ... These two qualities make polyacrylamide gelsthe preferred method for purifying significant quantities of small fragments.

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  • DETECTION OF MICROSATELLITE MARKERS IN

    2018-5-15 · polyacrylamide gel was modified from the two standard staining procedures. The main differences ... using a optimized method. The improved staining method we optimized is a modification of different ... Then 2000 ml cold (10-12°C)fixing solution The optimized methodwas fast (20 min) than

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  • Denaturing Polyacrylamide Gel Electrophoresis

    2019-2-5 · Place gel sandwich in electrophoresis apparatus and clamp plates to support. Sweep out any air bubbles at bottom of gel by squirting buffer between plates using syringe with a bent 20-G needle. 9. Pour 1× TBE buffer into top reservoir to ∼3 cm above top of gel. Rinse top of gel with 1× TBE buffer using a Pasteur pipet or Beral thin stem. 10.

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  • A novel method to make viscoelastic polyacrylamide

    2020-7-2 · Polyacrylamide (PAA) hydrogels are widely used to study in vitro the effect of the mechanical environment on cell behavior. 1–4 1. A. Engler, L. Bacakova, C. Newman, A. Hategan, M. Griffin, and D. Discher, “ Substrate compliance versus ligand density in cell on gel responses,” Biophys.J.

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  • Polyacrylamide Gel Polymer as Water Shut-off System ...

    2021-7-10 · polyacrylamide gel polymer using bottle testing method. Finally, the performance of the polyacrylamide gel polymer as water shut-off system was investigated in one of the Iranian oil reservoirs condition considering high temperature and high salinity. EXPERIMENTAL STUDIES Materials Partially hydrolyzed polyacrylamide was used as

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  • A method for sensitive staining of DNA in

    2013-6-24 · A visible dye-based staining method for DNA in polyacrylamide gels by ethyl violet. Anal. Biochem. 402,99–101 (2010).Crossref, Medline, CAS, Google Scholar; 12 Yang YI, Hong HY, Lee IS, Bai DG, Yoo GS, Choi JK. Detection of DNA using a visible dye, Nile blue, in electrophoresed gels. Anal. Biochem. 280,322–324 (2000).

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  • Affinity-Trap Polyacrylamide Gel Electrophoresis: A

    2009-12-28 · A method for the affinity capture of specific proteins from a complex mixture using a polyacrylamide gel technique is described. The approach is based on the orthogonal electro-transfer of proteins separated by ordinary polyacrylamide gel electrophoresis (PAGE) to a ligand-coupled polyacrylamide gel (Li-PAG), which is placed under the PAGE gel. Upon electro-transfer, the proteins …

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  • PURIFICATION OF OLIGONUCLEOTIDES USING

    1998-4-12 · Clean the gel plates thoroughly by washingthem with warm soapy water followed by an ethanol:water rinse.However, if the plates are particularly dirty or if the completeremoval of any residual nucleic acids is required, the platesmay be soaked in an 0.1 M NaOH for 30 minutes prior to washing.If the gel is particularly thin (<1 mm), silanizing ...

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  • The method of dehydration and purification of ...

    (57) Abstract: The method of dehydration and purification gel of polyacrylamide includes freezing, grinding and processing solvent. While remaining solvent from the dehydrated and purified particles are removed by a stream of air or inert gas at a temperature not exceeding 48 o C. expanding the number of solvents used for the dehydration and purification of the gel from impurities.

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  • Visible fluorescent detection of proteins in polyacrylamide ...

    2014-9-10 · 12% SDS–PAGE 1-mm thick gel as per the standard Laemmli method using the Protean II gel system from Bio-Rad Laboratories (Hercules, CA, USA) or the Atto Electrophoresis (AE-6450) Dual Mini Slab system (Atto Corp.). The low-molecular-weight standards from Bio-Rad Laboratories, contained phosphorylase b (97kDa,

    Get Price
  • DETECTION OF MICROSATELLITE MARKERS IN

    2018-5-15 · polyacrylamide gel was modified from the two standard staining procedures. The main differences ... using a optimized method. The improved staining method we optimized is a modification of different ... Then 2000 ml cold (10-12°C)fixing solution The optimized methodwas fast (20 min) than

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  • A Sensitive Fluorescent Method for the Detection of ...

    2019-1-29 · 24 hr and the concentration determined by the method of Lowry et al. (9) using bovine serum albumin as standard. Electrophoresis . Polyacrylamide gel electrophoresis was performed ac- cording to the method described by Reisfeld et al. (10). The gels were

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  • Blue Native Polyacrylamide Gel Electrophoresis

    2020-4-6 · 17. Carefully open gel cassette using a gel knife. To visualize the unstained marker, cut marker lane out as a strip and stain with 30 ml Coomassie Brilliant Blue R-250 stain solution. Always carefully handle the gel from the bottom; the top of the gradient gel is fragile. Staining can be greatly accelerated by microwaving at highest power for ...

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  • Method for drying polyacrylamide gel after

    1996-8-7 · A method for drying, while applying a pressure using a semipermeable film, a polyacrylamide gel which has been used for electrophoresis, comprising the steps of: replacing solution components contained in the gel by an aqueous solution containing one or more substances selected from the group consisting of saccharides, sugar alcohols having 4 ...

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  • Gel electrophoretic separation of proteins from

    Optimal separation of the sample was obtained using a 12% polyacrylamide gel. Furthermore, the use of silver staining allowed detection of proteins at nanogram levels, whereas for Coomassie Brilliant Blue staining, the detection limit was 10 times higher.

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  • BASIC PROTOCOL: PURIFICATION OF

    2016-8-9 · Tracking dyes suchas bromophenol blue and xylene cyanol (see table 2.12.1 for migration data)may be added to the samples or in empty lanes to monitor migration. 12. Electrophoresethe gel at 20-40 V/cm (constant voltage) until the tracking dyes indicatethat the oligonucleotide has migrated one-half to three-fourths the waythrough the gel.

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  • CA2183300A1 - Method for drying polyacrylamide gel

    A polyacrylamide gel which has undergone electrophoresis is dried in the presence of pressure applied via a semipermeable film placed on the gel. Before applying the semipermeable film onto the gel, solution components contained in the gel is replaced by an aqueous solution containing one or more substances selected from the group consisting of saccharides, sugar alcohols having 4 or more ...

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  • Degradable polyacrylamide gel - Case Western

    A degradable polyacrylamide gel for analyzing or separating at least one macromolecule in a sample using electrophoresis includes a polyacrylamide cross-linked with at least one degradable cross-linker having a ketal or acetal group with the formula (I), wherein R 1 and R 2 are the same or different and are hydrogen, an alkyl, or a substituted alkyl.

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  • SDS polyacrylamide gel electrophoresis: Trends in ...

    2000-12-1 · Fig. 1 SDS polyacrylamide gel electrophoresis of adenovirus type 2. (a) The first gel (7 July 1963) in which SDS was used. Adenovirus type 2 was dissociated with SDS and run on a 3% polyacrylamide gel containing 0.25% SDS in the Davis and Ornstein, high-pH, discontinuous buffer system, and stained with Amido Black.

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  • Polyacrylamide Gel Electrophoresis (Theory) :

    30% Polyacrylamide solution(29g acrylamide+1g bisacrylamide in 50 mL of water, dissolve completely using a magnetic stirrer, make the volume upto 100mL). …

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  • Fast and Efficient Elution of Proteins from Polyacrylamide ...

    2018-5-24 · SDS-PAGE using a PhastSystem (Pharmacia) and 12.5% polyacrylamide PhastGel (Pharmacia). The distance migrated by the protein was determined using a duplicate, silver stained gel. Then, a 3-mm strip of the gel containing the protein bands in six lanes was excised. Protein elution was performed using SDS elution buffer,

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  • A simple method for drying polyacrylamide slab

    A simple method for drying polyacrylamide slab gels using glycerol and gelatin. Electrophoresis, 1983. Octavian Popescu. Download PDF. Download Full PDF Package. This paper. A short summary of this paper. 37 Full PDFs related to this paper. READ PAPER.

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  • Nuclease detection in SDS-polyacrylamide gel ...

    2021-6-23 · @article{osti_7107068, title = {Nuclease detection in SDS-polyacrylamide gel electrophoresis}, author = {Rosenthal, A L and Lacks, S A}, abstractNote = {Nucleases can be revealed, following their electrophoretic separation in sodium dodecyl sulfate (SDS)-polyacrylamide gels containing DNA or RNA, by incubating the gels in buffer to remove SDS and to allow renaturation and enzymatic …

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  • Preparation of nano-TiO 2 powder by

    2009-7-25 · Polyacrylamide gel method is a new successful technique for preparing nanometer metallic oxide materials, such as ॅ-Al 2 O 3 , ZnO and ZrO 2 . TiO 2 made by this method, however, has not been reported. In this study, the nano-TiO 2 powder was prepared using the novel method with TiCl3 as raw materials, and with acrylamide and N,N'-methylenediacrylamide as crosslinking agent. The TG …

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  • Degradable polyacrylamide gel - Case Western

    2008-3-19 · A degradable polyacrylamide gel for analyzing or separating at least one macromolecule in a sample using electrophoresis includes a polyacrylamide cross-linked with at least one degradable cross-linker having a ketal or acetal group with the formula (I), wherein R 1 and R 2 are the same or different and are hydrogen, an alkyl, or a substituted alkyl.

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  • Preparation of nano-TiO 2 powder by

    Polyacrylamide gel method is a new successful technique for preparing nanometer metallic oxide materials, such as α-Al 2 O 3 , ZnO and ZrO 2 . TiO 2 made by this method, however, has not been reported. In this study, the nano-TiO 2 powder was prepared using the novel method with TiCl3 as raw materials, and with acrylamide and N,N'-methylenediacrylamide as crosslinking agent.

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  • Principle and Protocol of Sodium Dodecyl Sulphate ...

    2015-11-17 · Dilute 5-fold when using. The final concentration would be: Tris, 25mmol/L; glycine, 250mmol/L; SDS, 0.1% and the pH of the buffer is 8.3. 8. Polyacrylamide gel electrophoresis tank and electrophoresis power supply. 9. Transfer pipette and tip, etc. Operating Method. 1.

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  • [PDF] SDS-Polyacrylamide Gel Electrophoresis of

    INTRODUCTIONThis protocol describes the separation of proteins by SDS-polyacrylamide gel electrophoresis. SDS is used with a reducing agent and heat to dissociate the proteins. SDS-polypeptide complexes form and migrate through the gels according to the size of the polypeptide. By using markers of known molecular weight, the molecular weight of the polypeptide chain(s) can be estimated.

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  • Fabrication and photocatalytic performance of

    2020-7-22 · The authors have fabricated novel flower-like Bi 24 O 31 Br 10 micro/nanospheres by a polyacrylamide gel method. The crystal structure, microstructure, bandgap, morphology, and optical property of the as-prepared Bi 24 O 31 Br 10 micro/nanospheres were investigated by multiple techniques. The Bi 24 O 31 Br 10 photocatalyst exhibits excellent photocatalytic performance for the …

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  • Polyacrylamide gel electrophoresis using a 12 volt

    Polyacrylamide gel electrophoresis using a 12 volt battery charger as a power pack. High resolution microgels on a low budget JH Walker Biochemical Education 16(1): 43-44 1988. Please choose payment method: PayPal Credit Card (PDF emailed within 0-6 h: 19.90) Accession: 061999326 ...

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  • A simple method for drying polyacrylamide slab

    A simple method for drying polyacrylamide slab gels using glycerol and gelatin. Electrophoresis, 1983. Octavian Popescu. Download PDF. Download Full PDF Package. This paper. A short summary of this paper. 37 Full PDFs related to this paper. READ PAPER.

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  • <1056> BIOTECHNOLOGY-DERIVED ARTICLES

    Polyacrylamide gel electrophoresis (PAGE) is used for the qualitative characterization of proteins in biological preparations, for control of purity, and for quantitative determinations. This procedure is limited to the analysis of proteins with a weight range of 14,000 to 100,000 Da.

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  • Rapid detection of enteric adenovirus and rotavirus:

    A simple and rapid procedure for identifying adenovirus and rotavirus in stool extracts has been developed. The technique is based on polyacrylamide gel electrophoresis of the virus nucleic acid, but sample preparation is straightforward and does not entail phenol extraction or the use of a radioactive label. Furthermore, processing is not influenced by the amount of specimen obtained and is ...

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  • CA2183300A1 - Method for drying polyacrylamide

    A polyacrylamide gel which has undergone electrophoresis is dried in the presence of pressure applied via a semipermeable film placed on the gel. Before applying the semipermeable film onto the gel, solution components contained in the gel is replaced by an aqueous solution containing one or more substances selected from the group consisting of saccharides, sugar alcohols having 4 or more ...

    Get Price
  • Method for drying polyacrylamide gel after

    1996-8-7 · A method for drying, while applying a pressure using a semipermeable film, a polyacrylamide gel which has been used for electrophoresis, comprising the steps of: replacing solution components contained in the gel by an aqueous solution containing one or more substances selected from the group consisting of saccharides, sugar alcohols having 4 ...

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  • Detection of DNA in Polyacrylamide Gels by Staining

    Detection of DNA in Polyacrylamide Gels by Staining (Protocol summary only for purposes of this preview site) Unlike agarose gels, polyacrylamide gels cannot be cast in the presence of ethidium bromide because the dye inhibits polymerization of the acrylamide. However, ethidium bromide can be used to stain the polyacrylamide gel after ...

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  • Fast reversible single‐step method for enhanced

    2015-5-1 · To tackle these problems, we optimized and investigated a relatively obscure yet novel method of gel treatment that turns polyacrylamide gel opaque. Fadouloglou et al. first described a two‐step incubation to shrink and dry polyacrylamide gels using ethanol , developing this method further, others found the reaction to be reversible with ...

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  • Silver and Cyanine Staining of Oligonucleotides in ...

    2015-12-9 · To explore why some oligonucleotides in denaturing polyacrylamide gel could not be silver-stained, 134 different oligonucleotides were analyzed using denaturing polyacrylamide gel electrophoresis stained with silver and asymmetric cyanine. As a result, we found that the sensitivity of oligos (dA), (dC), (dG) and (dT) to silver staining could be ranged as (dA) > (dG) > (dC) > (dT) from …

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  • Mass spectrometric characterization of proteins ...

    2011-8-16 · The gel-resolved proteins can be characterized by MALDI-MS. Sloane et al. [] immobilized gel-resolved proteins onto Immobilon P SQ PVDF and nitrocellulose membranes, and digested the proteins with proteases on membranes using a piezoelectric chemical inkjet printer, and then they identified antigens and glycoproteins using MS. Kimura et al ...

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