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6 polyacrylamide gel in andorra

  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    2019-2-5 · Polyacrylamide Gel Electro-phoresis. If preformed-well comb was used, take care to prevent tearing of polyacrylamide wells. This comb will not be reinserted. 8. Fill bottom reservoir of gel apparatus with 1× TBE buffer so that gel plates will be submerged 2 to 3 cm in buffer. Place gel sandwich in electrophoresis apparatus and

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    2014-1-1 · 6.1. Safety Recommendations. Many of the reagents used during the processes described below are potentially dangerous. Acrylamide, acetone, isopropanol, silane, and N,N,N′,N′-tetramethylethylenediamine (TEMED) are very volatile compounds that should be handled under a chemical hood with adapted personal security protection (lab coat, gloves, protective glasses) and …

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  • Denaturing Polyacrylamide Gel Electrophoresis

    847 polyacrylamide absorbent gel products are offered for sale by suppliers on Alibaba.com A wide variety of polyacrylamide absorbent gel options are available to you, There are 47 suppliers who sells polyacrylamide absorbent gel on Alibaba.com, mainly located in Asia.

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  • Running agarose and polyacrylamide gels

    1981-1-1 · Acta histochem. 68, 227-230 (1981) Institute of Anatomy (Director: Prof. Dr. W. LnGet Price

  • Polyacrylamide Hydrogel Micropatterning -

    2019-7-22 · The slab gel was conditioned by applying 600 V for 1 h, with an upper (6.3 mM Tris-HCl, pH 7.4, 20 µM TPEN, 40 mM NaCl) and lower (6.3 mM Tris …

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  • polyacrylamide absorbent gel, polyacrylamide

    Recommended Polyacrylamide % for Separation in Denaturing Gels. % Acrylamide. Effective Range of Separation (nucleotides) 3.5. >500. 5. 151-500. 10. 61-150.

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  • Polyacrylamide Gel Electrophoresis | Cleaver Scientific

    2018-3-5 · Polyacrylamide Gel Electrophoresis (PAGE) When electrophoresis is performed in acrylamide or agarose gels, the gel serves as a size-selective sieve during separation. As proteins move through a gel in response to an electric field, the gel’s pore structure

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  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    2014-4-17 · 94 CHAPTER 6 Polyacrylamide Hydrogel Micropatterning. Microenvironment rigidity has also been shownto be akey parameter in the reg-ulation of several key physiological processes, including pathological ones (cell po- ... 6.4.3.3 Transfer on acrylamide gel 6.4.

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    There are two common types of gel: polyacrylamide and agarose. For most applications, denaturing acrylamide gels are most appropriate. These gels are extremely versatile and can resolve RNAs from ~600 to ≤20 nucleotides (nt). In certain circumstances, e.g., resolving different conformers of RNAs or RNA-protein complexes, native gels are ...

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  • CHAPTER Polyacrylamide Hydrogel Micropatterning 6

    2018-4-20 · Prepare a 6% non-denaturing polyacrylamide gel solution by dissolving 29 g of molecular biology grade acrylamide and 1 g of molecular biology grade N, N'-methylenebisacrylamide in 0.5× TBE buffer to a final volume of 500 mL. Cover the solution bottle with aluminum foil and

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  • Polyacrylamide Gel Electrophoresis of RNA

    1981-1-1 · Acta histochem. 68, 227-230 (1981) Institute of Anatomy (Director: Prof. Dr. W. LnGet Price

  • Markers using a Non-denaturing Polyacrylamide Gel A

    2015-8-1 · The most prevalent gel system was related to polyacrylamide (PAM), a synthetic polymer. Polysaccha- rides (biopolymers) such as starch, xanthan, guar, lignin and cellulose, and silicate gels with organic modification were also used in the oil industry. The raw materials, performance properties, and processing costs placed these polymers at a ...

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  • A polyacrylamide gel method for the cytochemical ...

    2016-3-22 · HPAAm gel could still keep intact after compressed to the strain of 0.9 while the corresponding polyacrylamide gel prepared by adding chemical crosslinker has been broken. Meanwhile, elongation at break of HPAAm gel could reach 1000%, which is also much higher than conventional polyacrylamide gels, indicating the high toughness of HPAAm gel.

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  • A comprehensive review of polyacrylamide polymer

    2014-1-1 · 6.1. Safety Recommendations. Many of the reagents used during the processes described below are potentially dangerous. Acrylamide, acetone, isopropanol, silane, and N,N,N′,N′-tetramethylethylenediamine (TEMED) are very volatile compounds that should be handled under a chemical hood with adapted personal security protection (lab coat, gloves, protective glasses) and …

    Get Price
  • CHAPTER Polyacrylamide Hydrogel Micropatterning 6

    2014-4-17 · 94 CHAPTER 6 Polyacrylamide Hydrogel Micropatterning. Microenvironment rigidity has also been shownto be akey parameter in the reg-ulation of several key physiological processes, including pathological ones (cell po- ... 6.4.3.3 Transfer on acrylamide gel 6.4.

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-3-5 · Polyacrylamide Gel Electrophoresis (PAGE) When electrophoresis is performed in acrylamide or agarose gels, the gel serves as a size-selective sieve during separation. As proteins move through a gel in response to an electric field, the gel’s pore structure

    Get Price
  • POLYACRYLAMIDE GEL ELECTROPHORESIS: A FASTER,

    2019-11-9 · Agarose gel electrophoresis run at 100-120V for 90 to 120 minutes with Ethidium Bromide staining and 6% Non-denaturing Polyacrylamide gel electrophoresis run at 110V for 90 minutes and silver stained. Gel fingerprints of agarose gel showed unseparated bands, with a lower throughput while gel fingerprints of polyacrylamide gel, showed clear ...

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  • 6A. Quality Control Check and Size Selection using

    2018-5-1 · Quality Control Check and Size Selection using 6% Polyacrylamide Gel NEBNext Small RNA Library Prep Set for Illumina (E7300, E7580, E7560, E7330) This protocol follows Protocol for use with NEBNext Small RNA Library Prep Set for Illumina E7300, E7580, E7560, E7335. 6A.1. Purify the PCR amplifed cDNA construct (100 µl) using a Monarch PCR & DNA ...

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  • Horizontal Polyacrylamide Gel Electrophoresis

    2015-8-1 · The most prevalent gel system was related to polyacrylamide (PAM), a synthetic polymer. Polysaccha- rides (biopolymers) such as starch, xanthan, guar, lignin and cellulose, and silicate gels with organic modification were also used in the oil industry. The raw materials, performance properties, and processing costs placed these polymers at a ...

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  • A comprehensive review of polyacrylamide polymer

    Polyacrylamide gel electrophoresis (PAGE) analysis can be conveniently applied to analyze the molecular weight of sulfated GAGs. Gels on which GAGs have been fractionated can be visualized with Alcian Blue with or without silver staining and the bands can be scanned and digitized. The average MW of a GAG is then calculated based on a mixture of HP-derived oligosaccharide standards prepared ...

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  • Polyacrylamide Gel Electrophoresis - an overview ...

    2016-3-22 · HPAAm gel could still keep intact after compressed to the strain of 0.9 while the corresponding polyacrylamide gel prepared by adding chemical crosslinker has been broken. Meanwhile, elongation at break of HPAAm gel could reach 1000%, which is also much higher than conventional polyacrylamide gels, indicating the high toughness of HPAAm gel.

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  • High-toughness polyacrylamide gel containing

    Elec-trophoresis 19: 1901. (8) GoKrg A, Boguth G, Obermaier C, Posch A and Weiss W (1995) Two-dimensional polyacrylamide gel elec-trophoresis with immobilized pH gradients in theRrst dimension (IPG-Dalt): the state of the art and the con-troversy of vertical …

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  • Two dimensional Polyacrylamide Gel

    Harrison, R. A. P., 1974, The detection of hexokinase, glucosephosphate isomerase and phosphoglucomutase activities in polyacrylamide gels after electrophoresis: a novel method using immobilized glucose-6-phosphate dehydrogenase, Anal. Biochem. 61:500. CrossRef Google Scholar

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  • Horizontal Polyacrylamide Gel Electrophoresis (hPAGE ...

    Bio-Gel P gels are autoclavable at pH 5.5–6.5 and operate over a pH range of 2–10 at room temperature. Flow rate and resolution increase with increasing temperature in the range of 4–80°C. The gels are compatible with dilute organic acids, 8 M urea, chaotropic …

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  • Polyacrylamide Gel Treatment of Antiretroviral

    2011-2-27 · Today, highly active antiretroviral therapy is lifesaving for most HIV-infected patients, but the treatment can result in facial lipoatrophy, which changes the face so radically that patients may develop severe psychological and social problems. Since 2001 polyacrylamide gel (PAAG) has been used successfully in HIV patients abroad. This article describes the results of a Danish study.

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  • Pre-Cast Gels for Gel Shift Assays | Thermo Fisher ...

    Performing Gel Shift Assays: The Novex ® 6% DNA Retardation Gels are used to perform gel shift assays. DNA Retardation Gels: Novex ® DNA Retardation Gels consist of 6% polyacrylamide prepared with 1/2X TBE as the gel buffer. The 6% gel provides good resolution of fragments in the range of 60-2500 bp used for DNA retardation assays.

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  • Global Polyacrylamide Gel Electrophoresis (PAGE)

    2021-7-7 · Polyacrylamide gel electrophoresis (PAGE) is a typical technique for protein separation by electrophoresis.In this technique, polyacrylamide gel is used as the base medium, while sodium dodecyl sulfate (SDS) is used for protein chain and protein denaturation. Market Analysis and Insights: Global ...

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  • The principle and method of polyacrylamide gel ...

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

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  • Conformation-sensitive gel electrophoresis for rapid ...

    The best separations of heteroduplexes and homoduplexes were obtained with a standard 6% polyacrylamide gel polymerized in 10% ethylene glycol/15% formamide/Tris-taurine buffer. As predicted by the hypothesis of solvent-induced bends, when the concentration of either ethylene glycol or formamide was increased, the differential migration decreased.

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  • Gel-Entrapment of Enzymes | SpringerLink

    2008-9-18 · 如题,'6% polyacrylamide sequencing gel' 是指什么样的聚丙烯酰胺凝胶?与一般的聚丙烯酰胺凝胶凝胶有什么不同吗? 回复此楼 » 猜你喜欢 求甲醇水溶液不同浓度下的表面张力,据说这边书里面 …

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  • LS23L Final - Lab 6: Polyacrylamide and Agarose Gel ...

    Harrison, R. A. P., 1974, The detection of hexokinase, glucosephosphate isomerase and phosphoglucomutase activities in polyacrylamide gels after electrophoresis: a novel method using immobilized glucose-6-phosphate dehydrogenase, Anal. Biochem. 61:500. CrossRef Google Scholar

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  • 6 polyacrylamide gel, 6 polyacrylamide gel Suppliers

    Alibaba.com offers 821 6 polyacrylamide gel products. A wide variety of 6 polyacrylamide gel options are available to you, such as type.

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  • Polyacrylamide Gel Electrophoresis (Theory) :

    Bio-Gel P gels are autoclavable at pH 5.5–6.5 and operate over a pH range of 2–10 at room temperature. Flow rate and resolution increase with increasing temperature in the range of 4–80°C. The gels are compatible with dilute organic acids, 8 M urea, chaotropic …

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  • 6 Polyacrylamide Gel Recipe | Bryont Rugs and Livings

    The gel used is divided into an upper 'stacking' gel of low percentage (with large pore size) and low pH (6.8), where the protein bands get squeezed down as a thin layer migrating toward the anode and a resolving gel (pH 8.8) with smaller pores. Cl-is the only mobile anion present in both gels. When electrophoresis begins, glycine present in ...

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  • Markers using a Non-denaturing Polyacrylamide Gel A

    2020-8-17 · Sds page acrylamide recipe 6 recipe for running portion of polyacrylamide gel volumes are protocol for making sds 2 separation polyacrylamide gel recipes for two gels. Share. Tweet. Google+. Pinterest. Email. Prev Article. Next Article . Related Articles.

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  • Denaturing Urea PAGE - Small Gel

    2018-4-20 · Prepare a 6% non-denaturing polyacrylamide gel solution by dissolving 29 g of molecular biology grade acrylamide and 1 g of molecular biology grade N, N'-methylenebisacrylamide in 0.5× TBE buffer to a final volume of 500 mL. Cover the solution bottle with aluminum foil and

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  • Reversible Functionalization of Clickable

    2001-6-22 · Denaturing Urea PAGE - Small Gel 1. Prepare denaturing polyacrylamide gel solution. Use Gibco/BRL apparatus. 7.2 % 9.6 % 12 % 10X TBE 2.5 mls 2.5 mls 2.5 mls Urea (ultrapure) 10.5 g 10.5 g 10.5 g 40% Acrylamide 4.5 mls 6 mls 7.5 mls ddH2O 10.5 mls 9 mls 7.5 mls Total Volume 25 mls 25 mls 25 mls Use 40% acrylamide stock for DNA/RNA gels.

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  • Silver staining of proteins in polyacrylamide gels ...

    2020-8-26 · The molecular mass of grafted copolymers (6.2 kDa) is estimated by monitoring the release process, expanding the tools available to characterize copolymers grafted onto hydrogels. Investigation of the efficiency of in-gel CuAAC reactions revealed limitations of the sequential modification approach, as well as guidelines to convert the singly ...

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  • Western Blotting Protocol for Characterizing

    2021-5-25 · Preparation of denaturing 6% polyacrylamide gels for microsatellite analysis (also for SSAP, high-resolution IRAP/ISSR and other analyses) Saadiah Jamli and Pat Heslop-Harrison November 2003 University of Leicester

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  • QC Check and Size Selection using 6% PolyAcrylamide

    2013-9-9 · QC Check and Size Selection using 6% PolyAcrylamide Gel - NEBNext Multiplex Small RNA Sample Prep Set for Illumina (E7300) Note: There are several different methods for performing size selection. It is recommended to choose the appropriate method based on the QC check of the library using the Bioanalyzer. Size selection using AMPure XP Beads ...

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  • 6A. Quality Control Check and Size Selection using 6% ...

    2018-5-1 · Quality Control Check and Size Selection using 6% Polyacrylamide Gel NEBNext Small RNA Library Prep Set for Illumina (E7300, E7580, E7560, E7330) This protocol follows Protocol for use with NEBNext Small RNA Library Prep Set for Illumina E7300, E7580, E7560, E7335. 6A.1. Purify the PCR amplifed cDNA construct (100 µl) using a Monarch PCR & DNA ...

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  • 652 - Precast Polyacrylamide Gels (6) - EDVOTEK

    Wego Chemical Group is an experienced importer of Polyacrylamide. Wego has the capability to stock and sell Polyacrylamide in numerous countries around the world. Wego is a supplier and distributor of Polyacrylamide worldwide. As a supplier of Polyacrylamide, Wego provides supply chain solutions to partners and customers in numerous industries.

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  • Polyacrylamide | Supplier & Distributor | CAS 9003-05-8

    2009-9-3 · Bio-Gel polyacrylamide gel Particle size Medium 90-180 µm Fine 45-90 µm Extra fine < 45 µm Shipping medium Shipped dry Resistance pH 2-10 Pressure 15 psi Organic solvents ... Bio-Gel P-6 Gel, Medium 90-180 6.5 15-20 1,000-6,000 Bio-Gel P-6 Gel, Fine 45-90 10.0-15 1,000-6,000 Bio-Gel P-6 Gel, Extra Fine < 45 <10 1,000-6,000 ...

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  • Native PAGE Gels | Thermo Fisher Scientific - IN

    Polyacrylamide gels are prepared using acrylamide and bis-acrylamide. Acrylamide is a water-soluble monomer, which forms a transparent stable insoluble gel. Bis-acrylamide is a cross-linking agent. Application. Acrylamide has been used: in polyacrylamide gel electrophoresis;

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  • Instruction Manual Polyacrylamide Gel

    Mono- and oligosaccharides, each containing a reducing end group, were labeled with the charged fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid and the resulting derivatives were separated with high resolution by polyacrylamide gel electrophoresis using a …

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  • Acrylamide for molecular biology, ≥99% (HPLC) | 79

    2018-8-21 · 6. Pour the acrylamide between the gel plates and insert the comb. Clamp the comb in place at the top of the gel to avoid separation of the gel from the plates as the acrylamide polymerizes. Allow the gel to polymerize for 30 min. Important note: polymerization begins as soon as APS is added to the mixture, so all subsequent steps must be

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  • Polyacrylamide gel electrophoresis of reducing

    2017-5-3 · Nagoya medical journal, 1999, 43(1): 1-6. [2]Dingman C W, Peacock A C. Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis[J]. Biochemistry, 1968, 7(2): 659-668.

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  • 652 - Precast Polyacrylamide Gels (6) - EDVOTEK

    2021-7-10 · Kansas by Willhite et al. [6]. Sydansk et al. [7] have reported several field tests of gel treatment for controlling of water production in Wyoming's Big Horn Basin. Recently, several gel polymer water (and gas) shut-off treatments have been experimented in the Middle East region [8,9]. Very few attempts have been made to

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  • Polyacrylamide Gel Polymer as Water Shut-off System ...

    The gel used is divided into an upper 'stacking' gel of low percentage (with large pore size) and low pH (6.8), where the protein bands get squeezed down as a thin layer migrating toward the anode and a resolving gel (pH 8.8) with smaller pores. Cl-is the only mobile anion present in both gels. When electrophoresis begins, glycine present in ...

    Get Price
  • Preparation of photodegradable polyacrylamide

    2006-11-22 · Schagger, H. & von Jagow, G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166 , …

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  • Polyacrylamide Gel Electrophoresis (Theory) :

    2001-6-22 · Denaturing Urea PAGE - Small Gel 1. Prepare denaturing polyacrylamide gel solution. Use Gibco/BRL apparatus. 7.2 % 9.6 % 12 % 10X TBE 2.5 mls 2.5 mls 2.5 mls Urea (ultrapure) 10.5 g 10.5 g 10.5 g 40% Acrylamide 4.5 mls 6 mls 7.5 mls ddH2O 10.5 mls 9 mls 7.5 mls Total Volume 25 mls 25 mls 25 mls Use 40% acrylamide stock for DNA/RNA gels.

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  • Silver staining of proteins in polyacrylamide gels ...

    2018-8-22 · Heat the gel (buffer) during the whole run at 60-70°C. 9. Wash the wells with 1X TBE buffer to remove UREA and gel pieces. 10. Load the samples. 11. Run the gel at 6 V/cm till the lower dye front reaches the three thirds of the gel. 12. Soak the gel for about 15 …

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  • Denaturing Urea PAGE - Small Gel

    Novex TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for g

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  • Denaturing Polyacrylamide/Urea Gel Electrophoresis

    Load the desired volume (e.g. 35 µL) of samples per well in a 10% polyacrylamide gel. Run SDS-PAGE for 30 minutes at 200 V. Rinse gel twice with distilled water. Incubate gel in cold Transfer Buffer for 15 minutes. IV. Immunoblotting and Imaging; Assemble transfer sandwich as per instrument instruction and then transfer the proteins onto a ...

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