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    2020-7-11 · Another low volume but important use of anionic and cationic polyacrylamides is gel electrophoresis for macromolecule separation. When an electic field is applied across a PAM gel, the (negatively) charged proteins or nucleic acids migrate across the gel away towards the positive electrode.

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    2015-11-23 · Product features: cationic polyacrylamide (CPAM) appearance of white powder, ionic degrees from 20% to 55% water soluble, can dissolve in any proportion of water and insoluble in organic solvents. The characteristics of high polymer electrolyte are suitable for the treatment of the wastewater containing negative charge and organic wastewater..

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    2021-7-13 · Full Article. A CATIONIC POLYACRYLAMIDE DISPERSION SYNTHESIS BY DISPERSION POLYMERIZATION IN AQUEOUS SOLUTION. Yufeng Wang, a,b, * Kefu Chen, a Lihuan Mo, a,* and Huiren Hu c A cationic polyacrylamide (CPAM) dispersion, the copolymer of acrylamide (AM) and acryloyloxyethyltrimethyl ammonium chloride (DAC), has been synthesized through dispersion …

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    2020-11-2 · Standard Gel staining Protocol 1- Gel may be prefixed in 50% MeOH, 10% HoAC, 40% H 2 O for 30 minutes to overnight. 2- Stain gel in the above solution, with 0.25-0.3% Coomassie Blue R-250, for 2 - 4 hours, until the gel is a uniform blue color. Staining is complete when the gel is no longer visible in the dye solution.

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    Novex® TBE-Urea Gels are denaturing polyacrylamide gels that resolve single-stranded DNA oligos or RNA into sharp, distinct bands. These gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase Protection Assays (RPA), in vitro transcription studies, and Northern blot analysis.

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    2020-11-2 · Standard Gel staining Protocol 1- Gel may be prefixed in 50% MeOH, 10% HoAC, 40% H 2 O for 30 minutes to overnight. 2- Stain gel in the above solution, with 0.25-0.3% Coomassie Blue R-250, for 2 - 4 hours, until the gel is a uniform blue color. Staining is complete when the gel is no longer visible in the dye solution.

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    As nucleic acids present strong negative charges in the neutral buffer solution while the mobility thereof depends on the molecular sieve effect of supportive matrix gels, either an about 0.3-2% agarose gel or an about 3.5-20% polyacrylamide gel is chiefly employed depending on the size of …

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    1986-7-1 · Above 20 mm free LDS, about 17 mg of LDS was bound per gram dry weight of polyacrylamide gel. This amount of binding might seem trivial. It is to be noted, however, that 10% polyacrylamide gel binds about 0.7 mg LDS per milliliter of gel in …

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    TAE buffer is a buffer solution containing a mixture of Tris base, acetic acid, and EDTA. In molecular biology, it is used in agarose electrophoresis typically for the separation of nucleic acids such as DNA and RNA. It is made up of Tris-acetate buffer, usually at pH 8.3, …

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    2019-2-1 · Cationic, anionic, nonionic, and zwitterionic acrylamide polymers and copolymers can be synthesized as desired. Anionic PAM is the most widely used reducer with excellent DR performance and low cost for hydraulic fracturing of shale gas formations. Polyacrylamide FRs are generally provided in dry powder and emulsion.

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