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    Cationic Polyacrylamide Emulsion with Ultra-high Concentration as a Flocculant for Paper Mill Wastewater Treatment. Kaiji Yang, a Jinghuan Chen, b and Chunli Yao a, * Cationic polyacrylamide emulsions prepared with ultra-high concentration (CPAME-uhc) have the advantages of fast dissolution, convenient operation, and low transportation cost.

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    Introduction: Polyacrylamide is a linear water-soluble polymer, and is one of the most widely used varieties of water-soluble polymer compounds.PAM and its derivatives can be used as efficient flocculants, thickeners, paper enhancers and liquid drag reducing agents, and Polyacrylamide are widely used in water treatment, paper making, petroleum, coal, mining, metallurgy, geology, textile ...

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    On a 15% polyacrylamide gel, these marker dyes co-migrate with oligonucleotides with lengths of 30 and 9–10 bases respectively. 6. Run the gel for 1.5 h at 200 V (for a minigel). When the dyes have migrated the desired distance, remove the gel and proceed with detection. (The gel may be run until the orange G dye has migrated out of the gel ...

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    15.1.3.3 Two-Dimensional Gel Electrophoresis. Two-dimensional gel electrophoresis (2DGel) is a successful method used for the detection and analysis of proteins. It has been designed as a combination of the 2DGel, IEF and SDS-PAGE methods, and is used in the analysis of complex protein mixtures. In the first step, protein is separated into its ...

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    Typically, two solutions are prepared: the light solution (equivalent to the lowest %T in the range to be poured) and a heavy solution (equivalent to the maximum %T to be poured). The most common gradient gel contains 4–20% acrylamide; however, the range of acrylamide concentrations should be chosen on the basis of the size of the proteins ...

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    Therefore, two different stiffnesses were studied—5% polyacrylamide gels have the lowest stiffness, around 1.5 kPa, while 7% polyacrylamide gels are stiffer, with values in the order of 15 kPa, according to the information from previous studies [25,26].

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    Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN ...

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    2018-1-1 · Polyacrylamide Gel Electrophoresis. Polyacrylamide gels are based on the free radical polymerization principle of acrylamide and cross-linking N,N′-methylene-bis-acrylamide. This material is physically very stable and strong. It is especially used for the electrophoretic separation of small or medium sized (up to about 1×10 6 Da) proteins ...

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    Typically, two solutions are prepared: the light solution (equivalent to the lowest %T in the range to be poured) and a heavy solution (equivalent to the maximum %T to be poured). The most common gradient gel contains 4–20% acrylamide; however, the range of acrylamide concentrations should be chosen on the basis of the size of the proteins ...

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    2017-10-10 · For the softer A8-B.10 gel at an exposure time of 300 s, the highest modulus was achieved at the lowest 15 mW/cm 2 UV light intensity; there was a 37% drop in modulus between gels made at 15 mW/cm 2 versus 131 mW/cm 2 intensity.

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    2019-6-27 · A Co x Mg 1–x Al 2 O 4 (x = 0.1–0.5) nanoparticles were prepared by an irradiation assisted polyacrylamide gel route. The mean particle size of the Co x Mg 1–x Al 2 O 4 photocatalysts increases with increase in the x value. The Co x Mg 1–x Al 2 O 4 photocatalysts possessed low Co content and exhibit a stabilize color (bright blue color) and optical properties. The Co x Mg 1–x Al 2 O ...

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    2016-3-7 · Then, the gel was fixed with 50% propanol, 5% acitic acid for 15 minutes. After 3 washes with MQ, the gel was incubated with fluorescent secondary …

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    2017-1-1 · For the softer A8-B.10 gel at an exposure time of 300 s, the highest modulus was achieved at the lowest 15 mW/[cm.sup.2] UV light intensity; there was a 37% drop in modulus between gels made at 15 mW/[cm.sup.2] versus 131 mW/cm intensity.

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