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  • Introduction to Polyacrylamide Gels | LSR | Bio-Rad

    Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling. Polyacrylamide gel electrophoresis of SDS-treated proteins allows researchers to separate proteins based on their length in an easy, inexpensive, and relatively accurate manner.

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  • The principle and Procedure of Polyacrylamide Gel ...

    2018-3-5 · a small-pore resolving gel, Figure 2.2) and different buffers in the gels and electrode solutions (Wheeler et al. 2004) In gel electrophoresis, proteins do not all enter the gel matrix at the same time. Samples are loaded into wells, and the proteins that are closer to the gel enter the gel first. In continuous systems, the uniform

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    A. Bianchi-Bosisio, in Encyclopedia of Analytical Science (Second Edition), 2005 Polyacrylamide Gel Electrophoresis. Polyacrylamide is an inert support whose porosity is easily adjusted by changing the composition of the acrylamide solution prior to polymerization. Although polyacrylamide gel electrophoresis (PAGE) is applicable to standard separations of native proteins, it can also be used ...

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  • The principle and method of polyacrylamide gel ...

    2015-3-1 · Recently, the inverse-suspension technique has been widely used for polyacrylamide-based hydrogels because of its easy removal and management of the hazardous, residual acrylamide monomer in the polymer. Fig. 5 represented the block diagram of suspension polymerization process for hydrogel production. Parameters critical to the preparation of hydrogel beads by suspension polymerization …

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    Polyacrylamide Gel. PA gels provide a variety of advantages including linear elastic material properties, reproducible rigidity, excellent optical qualities, capacity to covalently attach ECM molecules to an otherwise nonadhesive surface, and a porous nature to permit penetration of media and nutrients (Wang & Pelham, 1998).

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  • Polyacrylamide - an overview | ScienceDirect Topics

    GelGreen is a fluorescent nucleic acid stain designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. Pricing Match Criteria : …

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  • Preparation of protein samples for SDS-polyacrylamide

    2006-6-25 · Preparation of protein samples for SDS-polyacrylamide gel electrophoresis: procedures and tips Anthony C. Grabski 1and Richard R. Burgess2— Novagen, Inc. and 2McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706 continued from page 9 pensive method to maintain very low basal

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  • Polyacrylamide Gel Reagents | Life Science Research |

    Bio-Rad's acrylamide/bis solutions are made from 99.9% pure acrylamide and bis-acrylamide and are available in two concentrations, 30% and 40%, and three different crosslinker ratios, 19:1, 29:1 and 37.5:1. Acrylamide/bis solutions are provided ready to use and come with instructions. High-purity reagents and carefully controlled manufacturing ...

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  • Nucleic Acid Electrophoresis Workflow—5 Main Steps ...

    Five key steps in nucleic acid gel electrophoresis. 1. Selecting and preparing gels. Agarose and polyacrylamide are the two most common gel matrices utilized in the electrophoretic separation of nucleic acids. Both materials form 3-dimensional matrices with pore sizes appropriate for separation of nucleic acids and are nonreactive with the samples.

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  • ELECTROFORESIS EN GEL DE POLIACRILAMIDA CON SDS

    2019-1-20 · es el que emplea geles de poliacrilamida (PAGE = polyacrylamide gel electrophoresis) en presencia del detergente aniónico dodecilsulfato sódico (SDS). Esta técnica es conocida como SDS-PAGE. Para ello se prepara un gel en placa vertical. En la preparación del gel, la polimerización de los monómeros de acrilamida

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  • Synthesis of Hydrogel Nanocomposites Based on

    2020-3-5 · A recent gel process uses the preformed particle gels (PPGs) to overcome distinct drawbacks inherent in in-situ gelation systems, i.e. lack of control on gelation time, uncertain gelling due to shear degrdn., chromatog. fractionation or change of gel compns., and diln. by formation water.

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  • Ammonium persulfate for molecular biology, suitable

    Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN ...

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  • Super Absorbent Polymer Supplier - SOCO Polymer

    Polymerization. Thickening polymers are among the most-used ingredients in formulations. Because of their ability to govern rheological behavior, they are essential ingredients for controlling usage performance and ensuring users have a positive perception of the products. At Seppic, we use two main polymerization technologies: inverse emulsion ...

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  • Hydrogel: Preparation, characterization, and

    2015-3-1 · Recently, the inverse-suspension technique has been widely used for polyacrylamide-based hydrogels because of its easy removal and management of the hazardous, residual acrylamide monomer in the polymer. Fig. 5 represented the block diagram of suspension polymerization process for hydrogel production. Parameters critical to the preparation of ...

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  • Preparation of protein samples for SDS-polyacrylamide

    2006-6-25 · Preparation of protein samples for SDS-polyacrylamide gel electrophoresis: procedures and tips Anthony C. Grabski 1and Richard R. Burgess2— Novagen, Inc. and 2McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI 53706 continued from page 9 pensive method to maintain very low basal

    Get Price
  • The Polyacrylamide Matrix | National Diagnostics

    2012-7-23 · In an agarose gel, the pore size is large, so molecular sieving, i.e. separation by size, will not occur for smaller DNA fragments and most proteins. Additionally, by altering the total concentration of monomer in the gel and the ratio of acrylamide to bis, the pore size with a polyacrylamide gel can be altered in a reproducible manner.

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  • The Manufacturing Process Of Super Absorbent

    The invention concerns a novel composition for preparing a polyacryamide gel characterised in that it comprises a hydrophilic polymer which is not in the form of a dispersoid, methods for preparing polyacrylamide gels from said compositions and the use of said gels for separating nucleic acids by electrophoresis, in particular for sequencing and/or detecting nucleic acid mutation, separating ...

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  • Preparing SDS gels - Rice University

    2014-4-2 · Cross linking is a stabilization process in polymer chemistry which leads to multidimensional extension of polymeric chain resulting in network structure. Cross-link is a bond which links one polymer chain to other. It can be ionic or covalent. Cross linking changes a liquid polymer into ‘solid’ or ‘gel’ by restricting the ability

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  • WO2000077059A1 - Polyacrylamide gels

    2015-2-2 · 11. When the Stacking Gel has polymerized, remove the comb without distorting the shapes of the well. Remove the clips holding the plates together, and install the gel in the apparatus. 12. Fill apparatus with Reservoir Buffer. Push out the bottom spacer from the gel and remove bubbles from both the top and underneath of the gel. Use the gel ...

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  • Cross-linking in Hydrogels - A Review

    2020-3-5 · A recent gel process uses the preformed particle gels (PPGs) to overcome distinct drawbacks inherent in in-situ gelation systems, i.e. lack of control on gelation time, uncertain gelling due to shear degrdn., chromatog. fractionation or change of gel compns., and diln. by formation water.

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  • Molecular Techniques and Methods Native Gel

    Abstract. SDS-PAGE ( Chapter 11) is probably the most commonly used gel electrophoretic system for analyzing proteins.However, it should be stressed that this method separates denatured protein. Sometimes one needs to analyze native, nondenatured proteins, particularly if wanting to identify a protein in the gel by its biological activity (for example, enzyme activity, receptor binding ...

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  • Polyacrylamide Gel Reagents | Life Science Research |

    Bio-Rad's acrylamide/bis solutions are made from 99.9% pure acrylamide and bis-acrylamide and are available in two concentrations, 30% and 40%, and three different crosslinker ratios, 19:1, 29:1 and 37.5:1. Acrylamide/bis solutions are provided ready to use and come with instructions. High-purity reagents and carefully controlled manufacturing ...

    Get Price
  • Synthesis of Hydrogel Nanocomposites Based on

    2020-3-5 · A recent gel process uses the preformed particle gels (PPGs) to overcome distinct drawbacks inherent in in-situ gelation systems, i.e. lack of control on gelation time, uncertain gelling due to shear degrdn., chromatog. fractionation or change of gel compns., and diln. by formation water.

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  • Western Blot Technique: Principle, Steps, Uses •

    2009-7-24 · Cells on polyacrylamide gels of comparable low strain modulus to fibrin are round and thus display no orientation or alignment, making soft linear elastic gels an unsuitable control. As the cells appear to spread in accordance with a stiffer modulus, a 16 kPa fibrinogen-coated polyacrylamide gel was chosen as the control because hMSCs on this ...

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  • Non-Linear Elasticity of Extracellular Matrices Enables ...

    10 Prepare gels (6 % polyacrylamide; 8M urea) by mixing 60 ml of the urea: acrylamide solution in 10x TBE with 250 µl of ammonium persulfate and 50 µl of TEMED (Sigma). Apply the gel solution to the assembled gel plates (1.5 mm thick) using S2 Sequencing gel electrophoresis apparatus (Invitrogen). Allow the gel to polymerize during 30 min.

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  • Optimization of a reliable, fast, cheap and sensitive ...

    The process is short (about 15 minutes), and the gel can be photographed by viewing it over a dark background. Zinc staining is as sensitive as typical silver staining (detects less than 1 ng of protein), and no fixation steps are required. Furthermore, the stain is easily removed, making this method compatible with MS or western blotting.

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  • Protein Gel Staining Methods | Thermo Fisher

    Polyacrylamide gel provides a more precise resolution as compared to agarose gel making it more convenient for quantitative analysis. This gel helps to identify the binding process of proteins and DNA. It can also be used to understand how bacteria are becoming resistant to …

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  • Electrophoresis Market Size USD 4.21 Billion by 2028 ...

    A protein must be purified before its structure and the mechanism of its action can be studied. However, because proteins vary in size, charge, and water solubility, no single method can be used to isolate all proteins. To isolate one particular protein from the estimated 10,000 different proteins in a cell is a daunting task that requires methods both for separating proteins and for detecting ...

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  • Purifying, Detecting, and Characterizing Proteins ...

    PARAFFIN, 1899 TO 1914. Paraffin is a group of hydrocarbons, which is saturated with carbon to hydrogen bonds, making them relatively inert. The basic repeating unit in the polymers is: –(CH 2) n–.Paraffin exists as a hard form (wax) and a soft form (vaseline).

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  • Complications from injectable materials used for

    2021-7-1 · El asunto de los implantes de mama de silicona es una cuestión delicada y este debate, como muchos han dicho esta tarde, hace tiempo que debería haber tenido lugar. English It was lobbying from self-help groups of women suffering adverse effects from silicon- gel breast implants that prompted the Commission Communication.

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  • ELECTROFORESIS EN GEL DE POLIACRILAMIDA CON SDS

    2019-1-20 · es el que emplea geles de poliacrilamida (PAGE = polyacrylamide gel electrophoresis) en presencia del detergente aniónico dodecilsulfato sódico (SDS). Esta técnica es conocida como SDS-PAGE. Para ello se prepara un gel en placa vertical. En la preparación del gel, la polimerización de los monómeros de acrilamida

    Get Price
  • WO2000077059A1 - Polyacrylamide gels additionally ...

    The invention concerns a novel composition for preparing a polyacryamide gel characterised in that it comprises a hydrophilic polymer which is not in the form of a dispersoid, methods for preparing polyacrylamide gels from said compositions and the use of said gels for separating nucleic acids by electrophoresis, in particular for sequencing and/or detecting nucleic acid mutation, separating ...

    Get Price
  • Far-Western Blot Analysis | Thermo Fisher Scientific - US

    2020-3-5 · A recent gel process uses the preformed particle gels (PPGs) to overcome distinct drawbacks inherent in in-situ gelation systems, i.e. lack of control on gelation time, uncertain gelling due to shear degrdn., chromatog. fractionation or change of gel compns., and diln. by formation water.

    Get Price
  • Synthesis of Hydrogel Nanocomposites Based on

    The volume of gel you will need to make will depend on the size of the casting tray. For the smallest gel trays, 30-40mL is a convenient volume. The wells of the gel are made by inserting a comb into the slots in the tray, and as the agarose hardens around the comb, wells are formed. The thicker you pour your gel, the deeper the wells will be.

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  • How to make an agarose gel for electrophoresis

    2021-6-6 · Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) Market Size 2021 by Product Sales, Revenue, Price, Market Share, Growth Opportunity and Forecast to 2024 Research Report I Top key players-Bio-Rad Laboratories, Inc., General Electric, Merck KGaA, Thermo Fisher Scientific Inc., Rockland Immunochemicals, Inc., GenScript, Abcam ...

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  • Global Sodium Dodecyl Sulphate-Polyacrylamide Gel ...

    PARAFFIN, 1899 TO 1914. Paraffin is a group of hydrocarbons, which is saturated with carbon to hydrogen bonds, making them relatively inert. The basic repeating unit in the polymers is: –(CH 2) n–.Paraffin exists as a hard form (wax) and a soft form (vaseline).

    Get Price
  • western blotting power point presentation.

    Gelatin. Gelatin is a protein substance derived from collagen, a natural protein present in the tendons, ligaments, and tissues of mammals. It is produced by boiling the connective tissues, bones and skins of animals, usually cows and pigs. Gelatin's ability to form strong, transparent gels and flexible films that are easily digested, soluble ...

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  • Complications from injectable materials used for

    L'elaboració de formatge es pot considerar un 'procés controlat d'eliminació d'aigua de la llet'. Aquest procés concentra la proteïna, el greix i altres nutrients de la llet, augmentant la seva vida útil. La fabricació de formatge consta de diverses etapes d'entre les quals, dues de les més importants, tenen lloc en el tanc de formatgeria: la coagulació de la llet i la sinèresi de la ...

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  • Bio 6 – SDS-PAGE Lab

    2017-2-3 · polyacrylamide gel, stain the gel, and analyze the resulting bands of protein on the gel to estimate the molecular weight of each protein. Introduction SDS-PAGE is a very common laboratory technique used to analyze proteins. The acronym SDS-PAGE stands for sodium dodecyl sulfate – polyacrylamide gel electrophoresis. Sodium dodecyl sulfate or SDS

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  • ELECTROFORESIS EN GEL DE POLIACRILAMIDA CON SDS

    2019-1-20 · es el que emplea geles de poliacrilamida (PAGE = polyacrylamide gel electrophoresis) en presencia del detergente aniónico dodecilsulfato sódico (SDS). Esta técnica es conocida como SDS-PAGE. Para ello se prepara un gel en placa vertical. En la preparación del gel, la polimerización de los monómeros de acrilamida

    Get Price
  • How SDS-PAGE Works - Bitesize Bio

    2016-7-13 · How SDS-PAGE Works. SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) is commonly used in the lab for the separation of proteins based on their molecular weight. It’s one of those techniques that is commonly used but not frequently fully understood. So let’s try and fix that. SDS-PAGE separates proteins according to ...

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  • What is electrophoresis? | Cleaver Scientific

    2018-12-11 · Agarose gel is commonly used for electrophoresis of DNA. It has a large pore structure allowing larger molecules to move easily but it is not suitable for sequencing smaller molecules. Polyacrylamide gel electrophoresis (PAGE) has a clearer resolution than agarose gel making it more suitable for quantitative analysis. This makes it possible to ...

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  • Overview of Western Blotting | Thermo Fisher

    2020-6-3 · The fast and cost-effective DNA extraction is critical for all DNA-based detections. Here, we fabricated a new kind of polyacrylamide microsphere (PAMMP) in various sizes with two methods, spot polymerization (large size but low yield) and modified inverse microemulsion polymerization (small size but high yield). The fabricated PAMMPs have strong autofluorescence (fPAMMPs), including both ...

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  • Far-Western Blot Analysis | Thermo Fisher Scientific - US

    2019-12-12 · Rheological studies of low concentration (down to 0.1%) poly (acrylonitrile) (PAN)–dimethyl sulfoxide (DMSO) solutions revealed the existence of a rather unusual effect of the gel-like structure formation, while this phenomenon is absent at higher concentrations (17–24 wt %) of PAN in DMSO. The evidence of the gel-like structure consists in ...

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  • Fast DNA Extraction with Polyacrylamide

    Gels are a soft elastic material consisting of a three-dimensional polymer network with nanometer-sized pores and are used in a variety of applications. However, gel networks typically have a substantial level of defects because the network formation reaction proceeds stochastically. In this study, we present a general scheme to fabricate gels with extremely low levels of defects by applying ...

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  • Rheological Evidence of Gel Formation in Dilute Poly ...

    2013-8-29 · Gel Phase: Gel phase is a temperature phase during the soapmaking process. After soap is in the mold, the process of saponification can cause the soap to heat up. Gel phase is beneficial to soap because it can intensify colors in the soap and give soap a …

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  • Preparing protein samples for sds-page

    2012-11-18 · Polyacrylamide has a limited capacity for protein. ... and to adjust the concentrations of sample so that an appropriate amount of protein can be loaded onto a gel. ... Heating simply speeds up the process of denaturation by increasing molecular motion. It isn't necessary for some samples, but is necessary for membrane samples.

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  • Methods for Protein Analysis SDS-PAGE

    2014-9-22 · Methods for Protein Analysis 1. Protein Separation Methods The following is a quick review of some common methods used for protein separation: SDS-PAGE (SDS-polyacrylamide gel electrophoresis) separates proteins mainly on the basis of molecular weight as opposed to charge (which is ‘swamped out’

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  • Tough and stretchy double-network hydrogels

    2019-6-3 · Using super-tough thermoplastic polyurethane (HTPU) hydrogel and chemically cross-linked polyacrylamide (PAAm) as the first and second network, HTPU/PAAm double-network (DN) hydrogels are synthesized by a one-step radical polymerization in this research. The effects of the mass ratio of the two networks (HTPU and PAAm) and cross-linking density of the PAAm on the mechanical and …

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  • Polyacrylamide, Super Absorbent Polymer - Alibaba

    Wuxi Fengmin Environmental Technology Development Co., Ltd., Experts in Manufacturing and Exporting Polyacrylamide, Super Absorbent Polymer and 1085 more Products. A Verified CN Gold Supplier on Alibaba.com.

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  • LC3 and Autophagy - PubMed

    Microtubule-associated protein 1A/1B-light chain 3 (LC3) is a soluble protein with a molecular mass of approximately 17 kDa that is distributed ubiquitously in mammalian tissues and cultured cells. During autophagy, autophagosomes engulf cytoplasmic components, including cytosolic proteins and organ …

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  • DNA Gel Loading Dye (6X) - Thermo Fisher Scientific

    2016-1-1 · A number of parallel sequencing techniques sprung up following the success of 454. The most important among them is arguably the Solexa method of sequencing, which was later acquired by Illumina .Instead of parallelising by performing bead-based emPCR, adapter-bracketed DNA molecules are passed over a lawn of complementary oligonucleotides bound to a flowcell; a subsequent solid …

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  • The sequence of sequencers: The history of

    Registration is based on the ' one substance, one registration ' principle. This means that manufacturers and importers of the same substance have to submit their registration jointly. The analytical and spectral information provided should be consistent and sufficient to confirm the substance identity.

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  • Registration - ECHA

    JoVE publishes peer-reviewed scientific video protocols to accelerate biological, medical, chemical and physical research. Watch our scientific video articles.

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  • JoVE | Peer Reviewed Scientific Video Journal -

    Corning Introduces Revolutionary Fiber Designed to Accelerate Network Transformation to 5G, Cloud, and Beyond. 03-Jun-2021. Corning Incorporated (NYSE:GLW) today introduced its latest innovation, Corning SMF-28® Contour optical fiber, to help telecommunications operators address the ever-expanding number of connected devices, build-out of 5G ...

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