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polyacrylamide hydrolysis in cuba

  • Linking Mass Spectrometry and Slab-Polyacrylamide

    2019-5-25 · Haemophilus influenzae is an important cause of human disease, and its lipopolysaccharide (LPS) is known to be a major virulence factor. H. influenzae produces short-chain LPS of which the heterogeneity is often visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS−PAGE) using silver staining for detection.

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  • Enzyme activity and partial characterization of

    2017-12-12 · Polyacrylamide gel electrophoresis and zimogram. No-denaturing and denaturing polyacrylamide gel electrophoresis were performed in a Mini-PROTEAN Tetra Handcast Systems (Bio-Rad Laboratories, Hercules, CA, USA) according to Laemmli with a constant current of 24 mA for 60 min. The gels (15% acrylamide/bisacrylamide) were stained with Coomassie brilliant blue R-250.

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  • Interpenetrated Chitosan-Poly(Acrylic Acid-Co

    2013-12-24 · Interpenetrated polymer networks of chitosan (CHI), polyacrylic acid (PAA) and polyacrylamide (PAM) were prepared by free radical polymerization. These hydrogels were either washed with double distilled water (CHI/PAA/PAM) A or hydrolyzed with 1M sodium hydroxide (NaOH), (CHI/PAA/PAM) S. Both types of hydrogels were characterized by in-

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  • Purification and Characterization of Four New

    2007-10-12 · The precipitate was redissolved in deionized water. Polyacrylamide gels containing wide pH range ampholytes (3.0–10.0) were used. Focusing was developed under constant voltage conditions in a gradual procedure: 100 V for 30 min, 200 V for 15 min and 450 V for 60 min. The gel was stained with Coomassie Brilliant Blue R-250.

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  • Peptidases from Maclura Pomifera for Preparation of

    2020-10-13 · Tricine Sodium-Dodecyl Sulfate–Polyacrylamide-Gel Electrophoresis (SDS-PAGE) Hydrolysate samples prepared from the three substrates were analyzed by denaturing electrophoresis in tricine gels according to Reyes Jara et al. [g for 30 min. The gels containing 16% (w/v) polyacrylamide were runned for 2 h at 90 V, and afterwards stained with Coomassie brilliant blue G-250 to visualize …

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  • Complete sucrose hydrolysis by heat-killed

    2014-6-18 · Complete sucrose hydrolysis by the immobilized biocatalyst in batch and fixed-bed bioreactors. Batch hydrolysis of cane sugar (2.04 M) by the entrapped cells was conducted in a laboratory-scale stirred reactor operating at 60 and 70°C. A similar product profile was observed for the time-course experiments at the two temperatures (Figure 6). The complete sucrose inversion took 8 …

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  • Biochemical Aspects of a Serine Protease from

    2012-5-2 · The enzyme (50 nM) was kept in each pH for 10 min at 37°C. After that, the substrate (0.40 mM) was added and the hydrolysis in each pH was followed by the absorbance at 405 nm (ε pNA 8,990 M −1 cm −1 ) for 20 min in a Synergy HT microplate reader (purchased from Biotek Instruments, Winooski, VT, USA). For the thermal stability determination, the purified enzyme (50 nM) was first kept …

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  • A method for detection of cellulases in polyacrylamide ...

    1989-11-1 · The assay of endo-1,4-β-glucanases (cellulases) from Trichoderma reesei, T. longibrachiatum, and Sporotrichum pulverulentum by 5-bromoindoxyl-β-d-cellobioside is described.The substrate is enzymatically cleaved to afford 5-bromoindoxyl and latter undergoes immediate azo coupling with Fast Red or oxidation by nitroblue monotetrazolium chloride, various forms of endoglucanases …

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  • Cationic Polyacrylamide Less Price High Purity Fast

    From us, you can easily purchase Cationic Polyacrylamide at great prices. Place online order and we will dispatch your order through DHL, FedEx, UPS. You can also request for a quote by mailing us at [email protected] We invite you to contact us for further information about our …

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  • Aymé VALDÉS | Center of Molecular Immunology,

    In Cuba, little is known about antimalarial potentials of plant species used as medicinals. ... 103.1, 83.0, 71.7 and 45.4 kDa peptides was showed, using polyacrylamide gel electrophoresis in ...

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  • Interpenetrated Chitosan-Poly(Acrylic Acid-Co

    Acrylamide, also known as acrylic amide, is a white, odorless organic compound soluble in water and other organic solvents. Acrylamide can react violently when it is melted. Manufacturing process of acrylamide involves hydrolysis of acrylonitrile with the presence of a catalyst such as sulfuric acid and other metal salts.

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  • Acrylamide Market - Global Industry Analysis 2016 -

    2021-6-10 · Enzymatic hydrolysis is the method applied for dextran removal in the sugar industry (Li et al., 2017; Liu et al;, 2019b). Dextranase (α-d-1,6-glucan-6-glucanohydrolase; EC 3.2.1.11) catalyzes the random cleavage of α-(16) linkages in dextran polysaccharides to yield isomalto-oligosaccharides of two to five glucosyl units.

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  • Removal of bacterial dextran in sugarcane juice by ...

    Hydrolyzed collagen (HC) is a group of peptides with low molecular weight (3–6 KDa) that can be obtained by enzymatic action in acid or alkaline media at a specific incubation temperature. HC can be extracted from different sources such as bovine or porcine. These sources have presented health limitations in the last years. Recently research has shown good properties of the HC found in skin ...

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  • Molecules | Free Full-Text | Hydrolyzed

    2019-7-31 · 4Center of Protein Study, Facultad de Biolog´ıa, Universidad de la Habana, 10400 Havana, Cuba Correspondence should be addressed to Mariana da Silva Araujo,´ [email protected] Received 28 October 2011; Accepted 25 December 2011

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  • BiochemicalAspectsofaSerineProteasefromCaesalpinia ...

    1994-2-1 · Hydrolysis of modified chitosans Methylpyrrolidinone chitosan (obtained from the Katakura chitosan, lot no. B-00123-6) was submitted to hy- 1000 Viscosity, mPa.s 100 10 to Time, min loo Figure 3 Double logarithmic plot of viscosity versus time for chitosan (10 g I-') in lactate solution at pH 3.20 at 25 and 50 in the presence of immobilized ...

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  • Deploymerization of chitosan with the aid of papain ...

    2018-6-22 · The aim of this research is the partial characterization of proteases extracted from B. karatas; the isolation and purification of proteases from B. karatas fruits were achieved using precipitation, separation by size exclusion chromatography and anion-exchange chromatography; molecular mass (MM) was determined, and the effect of inhibitors, reducing agents and heat on enzyme activity was ...

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  • Development of new methods to study the amino

    2009-5-5 · protein extracted from the polyacrylamide gel is acylated with succinic or acetic anhydride. The mixture of tryptic peptides is re-acylated with an equimolar mixture of acetic normal and deuterated anhydrides. The N-term peptide is recognized in the MS spectrum because it is the only specie showing a normal isotopic distribution. 2.

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  • Nonionic Polyacrylamide Available Top Quality at

    2014-6-18 · Complete sucrose hydrolysis by the immobilized biocatalyst in batch and fixed-bed bioreactors. Batch hydrolysis of cane sugar (2.04 M) by the entrapped cells was conducted in a laboratory-scale stirred reactor operating at 60 and 70°C. A similar product profile was observed for the time-course experiments at the two temperatures (Figure 6 ...

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  • Complete sucrose hydrolysis by heat-killed

    After hydrolysis of the weak links in regime 1, we assume that the hydrolysis of the initially less accessible bonds triggers regime 2 (until ca. 192 h) with slower degradation of the remaining amorphous regions (a), characterized by a pseudo rate constant k ′ 2.

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  • Polymers | Free Full-Text | Biodegradation of

    2019-7-31 · 4Center of Protein Study, Facultad de Biolog´ıa, Universidad de la Habana, 10400 Havana, Cuba Correspondence should be addressed to Mariana da Silva Araujo,´ [email protected] Received 28 October 2011; Accepted 25 December 2011

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  • BiochemicalAspectsofaSerineProteasefromCaesalpinia ...

    2020-10-13 · Our objective was to isolate peptidases from the latex of Maclura pomifera fruits and use them to hydrolyze food proteins, as well as to purify and characterize the main peptidase. Two partially purified proteolytic extracts were prepared by ethanol (EE) and acetone (AE) precipitation from an aqueous suspension of exuded fruit latex. EE was used to hydrolyze food proteins with a ratio of 0.19 ...

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  • Peptidases from Maclura Pomifera for Preparation

    1994-2-1 · Hydrolysis of modified chitosans Methylpyrrolidinone chitosan (obtained from the Katakura chitosan, lot no. B-00123-6) was submitted to hy- 1000 Viscosity, mPa.s 100 10 to Time, min loo Figure 3 Double logarithmic plot of viscosity versus time for chitosan (10 g I-') in lactate solution at pH 3.20 at 25 and 50 in the presence of immobilized ...

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  • Deploymerization of chitosan with the aid of papain ...

    2021-6-10 · 1. Introduction. Dextran is a homopolymer (10 5 –10 7 Daltons) of d-glucopyranose units with around 95% α-(16) linkages and 5% branching through α-(12), α-(13) and/or α-(14) bonds (Wang et al., 2018).Sugar industries throughout the world are concerned about the economic losses caused by dextran, since its early and accumulative content in the sugarcane and sugar beet juices ...

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  • Removal of bacterial dextran in sugarcane juice by ...

    2021-5-10 · Protein immobilization by electrostatic adsorption to a support could represent a good option. On the other hand, lysozyme (EC 3.2.1.17) is a little and basic protein. The objective of this work was to test the functionality of the strategy of Rational Design of Immobilized Derivatives for the immobilization by electrostatic adsorption of egg white lysozyme on SP-Sepharose FastFlow support.

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  • Modeling and Experimental Validation of

    2018-7-1 · 1. Introduction. The Sabellastarte magnifica Carboxypeptidase Inhibitor (SmCI, UNIPROT ID: P84875) is the first natural inhibitor displaying dual A-like metallocarboxypeptidases (CPA) and serine proteases inhibition [].It is composed by 165 residues (19.7 kDa) arranged in three BPTI-Kunitz domains, which belong to the I2 family according to the MEROPS database classification [].

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  • Bioline International Official Site (site up-dated

    This is the active principle of the product registered in Cuba as Heberon alfa R ... Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed on 12.5% or 15% polyacrylamide gels, essentially as described by Laemmli [13]. ... Ile and Met can be explained on the basis of their chemical behavior during the acid hydrolysis ...

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  • Differential proteomic analysis of virus-enriched ...

    2017-8-29 · Following in-gel hydrolysis, peptides were analyzed by nano-liquid chromatography ... Additionally, reports from Cuba and Australia have sug-gested that circulating DV may become more virulent ... Denaturing polyacrylamide gel electrophoresis, Western blotting and silver-staining Following denaturation in SDS sample-buffer (Novex

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  • Purification and Characterization of Four New

    2007-10-12 · Bromelia pinguin L. is a plant broadly distributed in Central America and Caribbean islands. The fruits have been used in traditional medicine as anthelmintic, probably owed to the presence of a mixture of cysteine endopeptidases, initially termed pinguinain. This work deals with the purification and characterization of the four main components of that mixture, two of them showing acid pI and ...

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  • Increased levansucrase production by a genetically ...

    2019-5-1 · Measurement of a static polyacrylamide adsorbtion by optical and spectroscopic methods (Russian) L. A. Magadova; . L. A. Magadova

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  • Measurement of a static polyacrylamide adsorbtion by ...

    2006-10-5 · hydrolysis was stopped by incubating the mixture at 65 ºC for 12 min [15]. A sample without the nuclease treatment was used as the negative control. In all cases the sample was homogenized by vigorous stirring in a vortex for 5 min, or by sonication in a Branson 1200 ultrasonic batch, and filtered using 0.45 µm cellulose acetate filters.

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  • Purification of E. coli 055:B5 lipopolysaccharides by size ...

    2020-1-1 · Chemical hydrolysis has been used successfully in many marketed glycoconjugate vaccines, such as Menjugate and Meningitec, now manufactured by GSK and by Pfizer respectively (meningococcal serogroup C oligosaccharide conjugated to CRM197), Menveo by GSK (meningococcal serogroup A, C, W, Y oligosaccharides conjugated to CRM197) and Menactra by ...

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  • Glycoconjugate vaccines, production and

    After hydrolysis of the weak links in regime 1, we assume that the hydrolysis of the initially less accessible bonds triggers regime 2 (until ca. 192 h) with slower degradation of the remaining amorphous regions (a), characterized by a pseudo rate constant k ′ 2.

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  • Polymers | Free Full-Text | Biodegradation of

    Introduction. The Bromeliaceae family includes the Bromelia genus, which grows in neotropical zones in countries such as Mexico, Belize, Cuba, Puerto Rico, Guatemala, Panama, Brazil, the West Indies, and South America []. Bromelia karatas contains a good variety of minerals and vitamin C, and a high content of proteases [2, 3].. The number of proteases of plant origin that are used ...

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  • Enzyme activity and partial characterization of

    Due to the poor protein availability of algae in their unprocessed form, development of extraction methods for phycobiliproteins is of great significance. This study aimed to extract phycoerythrin (PE) and phycocyanin (PC) from Porphyra via bacterial enzymatic hydrolysis and to evaluate their bioactivity. To induce enzyme production, Porphyra powder was added into the culture medium of two ...

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  • A method for detection of cellulases in polyacrylamide ...

    1989-11-1 · The assay of endo-1,4-β-glucanases (cellulases) from Trichoderma reesei, T. longibrachiatum, and Sporotrichum pulverulentum by 5-bromoindoxyl-β-d-cellobioside is described.The substrate is enzymatically cleaved to afford 5-bromoindoxyl and latter undergoes immediate azo coupling with Fast Red or oxidation by nitroblue monotetrazolium chloride, various forms of endoglucanases …

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  • Hidrogeles superabsorbentes basados en

    2021-4-26 · Polyacrylamide hydrogels were produced by simultaneous synthesis and crosslinking, induced by gamma radiation in a 60Co irradiator, from a monomeric solution of 1, 26 M acrylamide. Obtained hydrogels are modified chemically by incomplete basic hydrolysis, using potassium hydroxide to achieve a poly (acrylamide-co-potassium acrylate) copolymer.

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  • Interpenetrated Chitosan-Poly(Acrylic Acid-Co

    2013-12-24 · Interpenetrated polymer networks of chitosan (CHI), polyacrylic acid (PAA) and polyacrylamide (PAM) were prepared by free radical polymerization. These hydrogels were either washed with double distilled water (CHI/PAA/PAM) A or hydrolyzed with 1M sodium hydroxide (NaOH), (CHI/PAA/PAM) S. Both types of hydrogels were characterized by in-

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  • (DOC) Impact of salts on polyacrylamide hydrolysis

    Impact of salts on polyacrylamide hydrolysis and gelation: New insights

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  • Linking Mass Spectrometry and Slab-Polyacrylamide

    2019-5-25 · Haemophilus influenzae is an important cause of human disease, and its lipopolysaccharide (LPS) is known to be a major virulence factor. H. influenzae produces short-chain LPS of which the heterogeneity is often visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS−PAGE) using silver staining for detection. Individual bands have not previously been recovered …

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  • Polymers Partial Hydrolyzed Polyacrylamide Phpa in

    PHPA Polymer,Partially Hydrolized Polyacrylamide,Drilling. It is a co-polymer of anionic character and high molecular weight. It is a water-soluble polymer, which is primarily used as a highly efficient fluid loss reducer, viscosifier and protective colloid for shale’s and cutting in fresh water, calcium, sodium brines and KCL mud systems.

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  • Interpenetrated Chitosan-Poly(Acrylic Acid-Co

    Anionic polyacrylamide as flocculants for water treatment 9003-05-8,It is widely used in Industrial wastewater;oil drilling/recovery, textile, wastewater treatment, paper -making industry, mine and metallurgy etc_OKCHEM

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  • Anionic polyacrylamide as flocculants for water

    2021-6-10 · Enzymatic hydrolysis is the method applied for dextran removal in the sugar industry (Li et al., 2017; Liu et al;, 2019b). Dextranase (α-d-1,6-glucan-6-glucanohydrolase; EC 3.2.1.11) catalyzes the random cleavage of α-(16) linkages in dextran polysaccharides to yield isomalto-oligosaccharides of two to five glucosyl units.

    Get Price
  • Removal of bacterial dextran in sugarcane juice by ...

    2017-12-12 · The enzymatic activity and partial characterization of proteases from Bromelia karatas fruits were evaluated and compared with Bromelia pinguin proteases. The specific activity increased twofold after partial purification in both proteases. Partially purified proteases from Bromelia karatas showed good specific activity at pH 6.0–8.0 and residual activity of 70–100% for 60 min at 37–60 ...

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  • Tri-domainBifunctionalInhibitorof ...

    2020-12-23 · Fromthe‡CentrodeEstudiodeProteínas,FacultaddeBiología,UniversidaddelaHabana,10400LaHabana,Cuba,the ... enzymes that catalyze the hydrolysis of peptide bonds at the C terminus of peptides and proteins. Besides a role in digestive ... The SDS-polyacrylamide gel was stained with Coomassie Blue R-250. …

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  • Development of new methods to study the amino

    2009-5-5 · protein extracted from the polyacrylamide gel is acylated with succinic or acetic anhydride. The mixture of tryptic peptides is re-acylated with an equimolar mixture of acetic normal and deuterated anhydrides. The N-term peptide is recognized in the MS spectrum because it is the only specie showing a normal isotopic distribution. 2.

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  • Production, purification, and characterization of a ...

    2007-4-17 · The activity corresponded to a single protein with an M r of 41.7 kDa according to sodium dodecyl sulfate polyacrylamide gel electrophoresis. The enzyme was stable in the pH range of 3.0–5.0 and displayed an optimal temperature of 55°C; it showed a typical endo-splitting way of substrate hydrolysis and exhibited a fair degree of activity on ...

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  • Antioxidant capacity of egg, milk and soy protein ...

    2018-6-6 · harvested in Cuba (Payrol et al., 2005, 2008). Degree of hydrolysis (DH) The DH ( Fig. 1) was carried out until 35 min since that after this time there was no significant difference (p<0.05). In all substrates, the B. pinguin proteases caused the highest DH. …

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  • Isolation and enzymic properties of levansucrase

    1995-7-1 · The purified protein consists of a single 58 kDa polypeptide with an isoelectric point of 5.5. Its activity is optimal at pH 5.0. It catalyses transfructosylation from sucrose to a variety of acceptors including water (sucrose hydrolysis), glucose (exchange reaction), fructan (polymerase reaction) and sucrose (oligofructoside synthesis).

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  • Adsorption of Cu, Cd, and Ni on Goethite in the

    2002-1-4 · The adsorption of copper, cadmium, and nickel on goethite was examined in natural groundwater samples from an infiltration site of the river Glatt at Glattfelden (Switzerland). Unfractionated dissolved organic matter was used at its natural concentrations. Metal concentrations were close to environmental conditions. Cu, Cd, and Ni presented the typical pH adsorption edge of cations. The …

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  • Actomyosin stress fiber mechanosensing in 2D and

    2016-9-7 · Actomyosin stress fiber mechanosensing in 2D and 3D. Stacey Lee1,2, Sanjay Kumar1-3. Stacey Lee 1,2 , Sanjay Kumar 1-3. PUBLISHED 07 Sep 2016. Author details. Author details. 1 Department of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA.

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  • Polymers | Free Full-Text | Biopolymer Electrolyte

    2018-6-18 · Related Links. Electrophoresis (Article in Press): 'Comparative two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE) of human milk to identify dysregulated proteins in breast cancer'. Electrophoresis, 2018, 39, 653–665: 'Proteomics analysis of human breast milk to assess breast cancer risk'. Article by Jon Evans. Read more about Jon and our other columnists >>>

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  • Antioxidant capacity of egg, milk and soy protein ...

    2018-6-6 · harvested in Cuba (Payrol et al., 2005, 2008). Degree of hydrolysis (DH) The DH ( Fig. 1) was carried out until 35 min since that after this time there was no significant difference (p<0.05). In all substrates, the B. pinguin proteases caused the highest DH. …

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  • Development of new methods to study the amino

    2009-5-5 · protein extracted from the polyacrylamide gel is acylated with succinic or acetic anhydride. The mixture of tryptic peptides is re-acylated with an equimolar mixture of acetic normal and deuterated anhydrides. The N-term peptide is recognized in the MS spectrum because it is the only specie showing a normal isotopic distribution. 2.

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  • Identification and Characterization of an Ecto ...

    2014-9-9 · In this study, we performed the molecular and biochemical characterization of an ecto-enzyme present in Trypanosoma rangeli that is involved with the hydrolysis of extracellular inorganic pyrophosphate. PCR analysis identified a putative proton-pyrophosphatase (H +-PPase) in the epimastigote forms of T. rangeli.This protein was recognized with Western blot and flow cytometry …

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  • Determination of Lysine with Ninhydrin-Ferric Reagent ...

    Table of contents – Volume 224, Issue 1 . Show all volumes and issues. The tables of contents are generated automatically and are based on the data records of the individual contributions available in the index of the TIB portal.

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  • Biochemical characteristics of a carbapenemase from

    1999-1-1 · Meropenem, 1 µM, achieved 50% inhibition of cephaloridine hydrolysis, indicating high affinity. The pI 6.9 enzyme was estimated to have a molecular mass of 30 kDa. The same method estimated the mass of TEM-1 enzyme as 36 kDa, 33% above the value from sequence data. 11 Imipenemase activity was not induced by growth with cefoxitin.

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  • Microbial Biomass in Batch and Continuous System |

    2002-1-4 · The adsorption of copper, cadmium, and nickel on goethite was examined in natural groundwater samples from an infiltration site of the river Glatt at Glattfelden (Switzerland). Unfractionated dissolved organic matter was used at its natural concentrations. Metal concentrations were close to environmental conditions. Cu, Cd, and Ni presented the typical pH adsorption edge of cations.

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  • Adsorption of Cu, Cd, and Ni on Goethite in the

    The mamey sapota (Pouteria sapota) is original from Mexico and Central America, but it is quite common in Cuba, North of South America and the West Indies. However, only after its introduction in Florida, mamey has become well-known and sought especially by Latin Americans.

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  • Viviane Nascimento - Academia.edu

    2014-5-7 · Skin Deep: Fibroblast Proteome Holds Clue to Pathogenesis of Triglyceride Deposit Cardiomyovasculopathy. By Amanda Maxwell 05.07.2014. In their recent clinical paper, Hara and co-authors describe their initial steps in untangling the pathogenesis of cardiac failure due to abnormal triglyceride deposition. They accomplished this by cellular ...

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  • Skin Deep: Fibroblast Proteome Holds Clue to

    Through this chitin‐binding activity, Avr4 was found to protect C. fulvum hyphae from hydrolysis by plant chitinases (van den Burg et al., 2006; van Esse et al., 2007). It is tempting to speculate that C. fulvum Ecp6 is a chitin‐binding protein too.

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