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polyacrylamide tga in italy

  • Polyacrylamide hydrogel application in sand control with ...

    2019-1-12 · along with thermogravimetric analysis (TGA) were per-formed. Also, the effect of mono and divalent cations and temperature on the hydrogel stability was studied. 2 Experimental 2.1 Materials Hydrolyzed acrylamido propyl sulfonated acid (polyacry-lamide polymer sodium salt, trade name of AN125, molecular weight (MW) of 8.0 9 106, sulfonation degree

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  • Polyacrylamide hydrogel application in sand control with ...

    Finally, the thermal properties of the nanohybrid were evaluated by using thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The results showed that the CSNH-PC1 has a spherical morphology, with sizes between 420–480 nm and higher thermal resistance compared to HPAM polymers without modification, with a glass transition temperature of 360 …

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  • New Nanohybrid Based on Hydrolyzed Polyacrylamide and ...

    2020-5-18 · nanoparticles (nSiO2-APTES), and the hydrolyzed polyacrylamide (HPAM) chain (MW ˇ 20.106Da) was confirmed with high-resolution X-ray spectroscopy (XPS). Finally, the thermal properties. of the nanohybrid were evaluated by using thermogravimetric analysis (TGA) and di erential. scanning calorimetry (DSC).

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  • New Nanohybrid Based on Hydrolyzed Polyacrylamide and ...

    2017-4-20 · Polyacrylamide (PAM) hydrogels with different crosslinking degree are synthesized and studied in this work by different analytical techniques. Swelling properties are characterized in water and in different aqueous salt solutions, and the results are correlated with the dynamic light scattering measurements of swollen hydrogels; it is demonstrated that gels are more swollen in …

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  • Polyacrylamide Hydrogel: An Alternative Osteoarthritis ...

    1994-12-30 · Electrophoresis was performed in an8% polyacrylamide gel containing 7 M urea in a Sequi-Gen Nucleic Acid Sequencing Cell from Bio-Rad (Richmond, CAI. After electrophoresis, the gel was fixed in 10% acetic acid, 10% methanol and dried in a Slab Gel Dryer SE 1160 (Hoefer Scientific Instruments, San Francisco, CA) at 80 'C. Radiolabeled bands were visualized by …

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  • Polyacrylamide hydrogels for stone restoration: Effect of ...

    specific record if its presence in Italy seems to exist. The virus is seed transmitted and is spread by the weevels Apion vorax Hbst. and Sitona lineatus L. Of these A. vorax seems to be a more efficient vector than S. lineatus (Cockbain et al., 1975). Both species have been recorded in Italy, but in Apulia it appears that only S. lineatus is present

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  • A frameshift mutation in Exon V of the A alpha-chain gene ...

    2019-4-18 · The PAM-PASS was prepared at the optimal conditions mentioned above and used for the treatment of waste water. The rates of turbidity and color removal were 99.6% and 98.2%. Place the PAM - PASS in the air for 100 days the rate of deturbidity in a more than 90%. Respectively, when the dosage of PAM-PASS was 15 mg/L.

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  • Virus diseases of vegetable crops in Apulia. XXIV. True ...

    Several analytical techniques–some non-invasive such as Fourier-transform infrared spectroscopy (FTIR) and portable magnetic resonance (unilateral NMR), joined to micro-destructive ones such as scanning electron microscopy (SEM), 13 C CPMAS NMR spectroscopy, thermo-gravimetric analysis (TGA), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and reverse …

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  • Use of polymers and biopolymers for water retaining and ...

    2010-1-1 · Polyacrylamide is a long-chain synthetic polymer that acts as a ... Both thermo gravimetric analysis (TGA) and differential scanning calorimetry (DSC) of all samples were performed up to a temperature of 600°C, starting from room temperature in nitrogen atmosphere. ... (institute of chemistry and technology of polymers-Pozzuoli Italy) for ...

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  • Polyacrylamide hydrogel application in sand control with ...

    2019-1-12 · Polyacrylamide hydrogel application in sand control with compressive ... (TGA) were per-formed. Also, the effect of mono and divalent cations and temperature on the hydrogel stability was studied. ... Simax, Italy) and were kept at 90 C in an UN55 oven (Memmert, Germany). The glass tubes were inverted at

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  • Graphene Improves the Biocompatibility of

    2017-9-8 · Preparation of the pure polyacrylamide hydrogel (AM) Pure polyacrylamide hydrogels were prepared using Am, MBA as cross-linker and KPS as initiator. Am was initially dissolved in ultrapure Milli-Q water (200 mg mL −1) together with the MBA (0.2 mg mL −1). The solution was homogenised by stirring and mild sonication.

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  • Polyacrylamide Hydrogel: An Alternative Osteoarthritis ...

    The dispute over the paternity of western paper is a longstanding problem. In Italy, a country that has a long tradition of papermaking, many cities compete for the leadership in the production of this material; on the other hand, several studies attribute to Fabriano (13th century), a little center in Marche region, the birth of modern paper and its diffusion throughout Europe.

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  • Characterization of Handmade Papers (13th–15th century ...

    An inorganic-organic hybrid flocculant Al(OH) 3-polyacrylamide (Al-PAM) with narrow molecular weight distribution was synthesized using inverse microemulsion po

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  • characterization of a hybrid polyacrylamide and its in ...

    2017-9-8 · A chemically cross-linked polyacrylamide gel (AM) prepared without graphene and a series of hybrid graphene-polyacrylamide gels (AMGX, being X the graphene concentration used in mg mL −1) were ...

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  • Graphene Improves the Biocompatibility of

    TGA mmol), dissolved in THF, (4 mL) and was added drop wise measurements were performed using a TA Instrument ther- by means of an addition funnel. The reaction mixture was mobalance TGA 2050, under air flow, from 25 to 500 C at a stirred for 24 h at room temperature.

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  • (PDF) Synthesis and characterization of polyethylene ...

    the speci¢c primers for rat VEGF: 5P-CCA TGA ACT TTC TGC TCT CTT G-3P (nucleotides 2^24) and 5P-GGT GAG AGG TCT AGT TCC CGA-3P (nucleotides 610^630) [14] by Taq DNA polymer-ase (Promega, Milan, Italy) in 25 Wl of standard buer (10 mM Tris-HCl, pH 9, 50 mM KCl, 0.1% Triton X-100, 2.5 mM MgCl2, and 200 WM dNTPs).

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  • Polyacrylamide Hydrogel: An Alternative Osteoarthritis ...

    2017-9-8 · A chemically cross-linked polyacrylamide gel (AM) prepared without graphene and a series of hybrid graphene-polyacrylamide gels (AMGX, being X the graphene concentration used in mg mL −1) were ...

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  • Graphene Improves the Biocompatibility of Polyacrylamide ...

    2021-3-13 · NucleicAcidsResearch kinase and gamma-2P-ATP and purified by fractionation in a 20%polyacrylamide/urea gel, and elution of the radioactive band at 370C, overnight. One pmole of each labelled oligonucleotide was hybridized to 20 ug of HeLa polyA+ RNA in Pipes-NaCl buffer (10mM Pipes, pH 6.9, 400mM NaCl, lmM EDTA). Hybridization was for 18 hours at 600C for oligo …

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  • International Institute Genetics Biophysics, CNR, Via ...

    2019-9-9 · Europe PMC is an archive of life sciences journal literature. Two series of novel dumbbell-shaped polyhedral oligomeric silsesquioxanes (POSSs), fully functionalized with phenyl groups at the corner of the silicon cages, were used to prepare polystyrene (PS) nanocomposites through the method of in situ polymerization.

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  • Polystyrene Nanocomposites Reinforced with Novel

    Fanconi anemia (FA) is an autosomal recessive disorder exhibiting chromosomal fragility, bone-marrow failure, congenital abnormalities, and cancer. At least eight complementation groups have been described, with group A accounting for 60%–65% of FA patients. Mutation screening of the group A gene (FANCA) is complicated by its highly interrupted genomic structure and …

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  • Characterization of mutations in ATP8B1 associated with ...

    The incidence of S. pyogenes erythromycin resistance remains low in most parts of the world but recently reached 17% in Finland , 27 to 34% in Spain (2, 32, 33), and 30 to 35% in Italy (5, 11). In our study, the prevalence of erythromycin resistance in S. pyogenes isolates from French children was only 6.2%. Similar percentages (4 to 10%) have ...

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  • High Frequency of Large Intragenic Deletions in the ...

    2008-6-28 · Solanum nigrum plant height and abundance of viral RNA after treatment with different silencing vectors. (a) Mean (± SE) heights of LAP-silenced plants (B-LAP) and of plants treated with two different control vectors: empty vector B2 (B-EV), and vector B2 with an insert of intronic PDS sequence (B-CV). Untreated wild-type (WT) and wild-type plants infiltrated with untransformed …

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  • Resistance to Macrolides in Streptococcus pyogenes in ...

    2009-11-9 · The outer membrane proteins YadA and invasin of Yersinia pseudotuberculosis promote invasion into mammalian cells through β 1 ‐integrins and trigger the production of interleukin (IL)‐8. FAK, c‐Src and the PI3 kinase were previously found to be important for both YadA‐ and invasin‐promoted uptake.

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  • Optimized virus‐induced gene silencing in Solanum nigrum ...

    2020-9-30 · Expression of the axon-guidance gene Slit2 in endothelium, induced by endothelial sensing of tumour-derived double-stranded RNA, promotes metastatic dissemination in mouse models of breast and ...

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  • Phyto85n06 728 - APS Home

    2018-12-16 · Italy. Direct PCR amplification with A, the universal primer pair R16F1/R0 and B, the universal primer pair R16F2/R2 and nested PCR assays with C, the primer pair R16F1/R0 followed by R 16F2/R2 and D, the primer pair R 16Fl/RO followed by modified R 16F2/R2. S DNA fragment sizes standard; Control-w

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  • AFLP™ markers for DNA fingerprinting in cattle

    2018-1-7 · 4·5% polyacrylamide sequencing gel in TBE buffer (100 mM Tris-HCl, 100 mM Boric acid, 2 mM EDTA). Gels were run at constant wattage (120 W). Following the run, the gel was fixed for 20 min in 10% acetic acid, rinsed with deion-ized water, dried and exposed to Fujix Phosphorimage screens (Raytest, Isotopen-messgeräte, Straubenhardt, Germany ...

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  • Supplemental Information CD44v6 Is a Marker of ...

    2015-1-31 · TGA AGA CAT CTA CCC CAG CAA CCC TAC TGA TGA TGA CGT GAG CAG CGG ... River Laboratories (Milan, Italy) were maintained in accordance to the institutional ... on SDS-polyacrylamide gels and blotted on nitrocellulose membranes, as previously described (Lombardo et al., 2011). Membranes were exposed to specific antibodies for

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  • Characterization of Fibroblast Growth Factor Receptors ...

    2003-6-1 · Forty micrograms of protein in Laemmli sample buffer (60 mM Tris HCl, pH 6.8, 2% SDS, 10% glycerol, 0.1% bromophenol blue, and 5% β-mercaptoethanol) was loaded onto 6% SDS-polyacrylamide gels and transferred onto 0.45-μm nitrocellulose membranes (Bio-Rad) in 25 mM Tris base, 192 mM glycine, and 20% methanol at 4°C for 1 h at 100 V. Prior to ...

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  • HLA‐DQ genotype and biochemical characterization of anti ...

    2020-5-3 · TGM2 is the predominant autoantigen in CD and serologic screening of anti-TGM2 IgA (TGA) (or anti-TGM2-IgG; TGG) is the standard test for CD as its specificity in predicting CD. 8 TGM2 deamidates Gln (Q) to Glu (E) in the gliadin which increases its affinity to the HLA-DQ2 or HLA-DQ8 molecules on antigen-presenting cells. 1 Then antibodies ...

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  • Resistance to Macrolides in Streptococcus pyogenes in ...

    The incidence of S. pyogenes erythromycin resistance remains low in most parts of the world but recently reached 17% in Finland , 27 to 34% in Spain (2, 32, 33), and 30 to 35% in Italy (5, 11). In our study, the prevalence of erythromycin resistance in S. pyogenes isolates from French children was only 6.2%. Similar percentages (4 to 10%) have ...

    Get Price
  • Charge-based precipitation of extracellular vesicles

    2016-9-29 · Vesicular-mediated communication between cells appears critical in many biological processes. Extracellular vesicles (EVs) released from healthy and diseased cells are involved in a network of exchange of biologically active molecules. Since EVs present in biological fluids carry the signature of the cell of origin, they are potential biomarkers for ongoing physiological or …

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  • Optimized virus‐induced gene silencing in Solanum nigrum ...

    2008-6-28 · Solanum nigrum plant height and abundance of viral RNA after treatment with different silencing vectors. (a) Mean (± SE) heights of LAP-silenced plants (B-LAP) and of plants treated with two different control vectors: empty vector B2 (B-EV), and vector B2 with an insert of intronic PDS sequence (B-CV). Untreated wild-type (WT) and wild-type plants infiltrated with untransformed …

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  • Redox Modification of Cysteine Residues Regulates the ...

    2021-1-11 · CCA CCT CTC TGA GGC CTT CTT GGA GAA CTC-3′. The PCR product was subcloned into the pCAL-n vector, 3′to the T7 promotor and CBP tag, expressed and purified as described previously (2). DNase I was added at 100 U/mL to the beads to remove any contaminating DNA. Degradation of DNA was verified by ethidium bromide staining of agarose

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  • Role of Ionic Moieties in Hydrogel Networks to Remove ...

    2021-1-12 · The thermal stability of hydrogels was analyzed using a thermogravimetric analyzer (DT/TGA 7200, HITACHI, Japan). Approximately, 5 mg of the previously dried and the powdered sample was taken in a platinum pan for each test and analyzed from 25 to 800 °C at a heating rate of 10 °C/m under a constant flow of nitrogen at a flow rate of 10 mL/m.

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  • AFLP™ markers for DNA fingerprinting in cattle

    2018-1-7 · 4·5% polyacrylamide sequencing gel in TBE buffer (100 mM Tris-HCl, 100 mM Boric acid, 2 mM EDTA). Gels were run at constant wattage (120 W). Following the run, the gel was fixed for 20 min in 10% acetic acid, rinsed with deion-ized water, dried and exposed to Fujix Phosphorimage screens (Raytest, Isotopen-messgeräte, Straubenhardt, Germany ...

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  • Molecular Analysis of the ERGIC-53 Gene in 35 Families ...

    1999-4-1 · The other two, R202X and R456X, were CGA to TGA mutations in accordance with the known CpG to TpG hypermutability.13 The R456X was found in both Chinese and Pakistani families on a different haplotype (A19 homozygous for R117R cgg; A27 homozygous for R117R cga) showing recurrence of this mutation.

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  • Australian public assessment report for nanoparticle ...

    Cancer of the exocrine pancreas is a highly lethal malignancy, with a five-year survival rate of fewer than 5%. It is the fifth leading cause of cancer-related death in Australia (Cancer Council Australia) accounting for 5% of all cancer deaths, and second only to colorectal cancer as a cause of digestive cancer-related death.

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  • Role of Ionic Moieties in Hydrogel Networks to Remove ...

    2021-1-12 · The thermal stability of hydrogels was analyzed using a thermogravimetric analyzer (DT/TGA 7200, HITACHI, Japan). Approximately, 5 mg of the previously dried and the powdered sample was taken in a platinum pan for each test and analyzed from 25 to 800 °C at a heating rate of 10 °C/m under a constant flow of nitrogen at a flow rate of 10 mL/m.

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  • HDI Based Poly(Urethane Methacrylate) Nanocomposites ...

    2021-3-26 · (CEAST, Italy AQ 18). Rectangular bar specimen of 63.5x12.7x3mm dimension with a V-notch depth of 2.54mm and a notch angle of 45° was used for the determination of impact strength. Five samples were tested, and the average was taken for tensile, flexural and impact properties. Abrasion resistance was determined as per ASTM

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  • Preparation of DNA-crosslinked Polyacrylamide Hydrogels ...

    C111 or 22589. Prepare a 0.315 mg/ml solution in water immediately before use. Dissolve at 37 °C and filter sterilze. It is normal to observe undisolved sulfo-SANPAH in the filter. Sulfo-SANPAH is light sensitive and, therefore, the solution should be protect from light until UV exposure.

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  • Fluorescent Visualization of Mango-tagged RNA in ...

    A significant advantage of the Mango fluorescent tag is that a single tag can be used in multiple ways. The high brightness and affinity of these aptamers make them useful not only for in cell visualization 2 but also for in vitro RNA or RNP purification 4.Therefore, gel ...

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  • Pluronic® F-127 powder, BioReagent, suitable for cell ...

    A non-ionic copolymer surfactant qualified for use in insect cell culture applications as an antifoaming agent. Pluronic® F-127 was used to coat a siliconized coverslip to hold an egg extract in a study. Pluronic F-127 was added to phosphate buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device.

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  • Existence and functions of a kisspeptin neuropeptide ...

    2020-6-9 · The putative A. japonicus kisspeptin precursor gene (AjpreKiss) was identified in silico from transcriptome data and cloned from anterior part (ANP, containing the nerve ring) tissue samples by reverse transcription polymerase chain reaction (RT-PCR).The full-length cDNA (GenBank accession number MH635262) was 2481-bp long and contained a 543-bp open …

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  • Re-evaluation of the nor mutation and the role of the NAC ...

    Introduction. Plant fruits protect developing seeds and aid in their dissemination (Forlani et al., 2019).They are also an important food source for humans and animals and are rich in nutrients such as carbohydrates, fats, proteins, vitamins, and trace elements (Giovannoni, 2004; Klee and Giovannoni, 2011).Fleshy fruit ripening and the generation of quality attributes occur towards the …

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  • Molecular Analysis of the ERGIC-53 Gene in 35 Families ...

    1999-4-1 · The other two, R202X and R456X, were CGA to TGA mutations in accordance with the known CpG to TpG hypermutability.13 The R456X was found in both Chinese and Pakistani families on a different haplotype (A19 homozygous for R117R cgg; A27 homozygous for R117R cga) showing recurrence of this mutation.

    Get Price
  • Immune reaction against the cytoskeleton in coeliac ... - Gut

    2021-7-13 · BACKGROUND The cytoskeleton actin network of intestinal microvilli has been found to be rapidly impaired after gluten challenge in coeliac disease (CD). The aim of this study was to investigate the presence of an immune reaction towards cytoskeleton structures such as actin filaments in CD. METHODS Eighty three antiendomysial antibody positive CD patients (52 …

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  • Pluronic® F-127 powder, BioReagent, suitable for cell ...

    A non-ionic copolymer surfactant qualified for use in insect cell culture applications as an antifoaming agent. Pluronic® F-127 was used to coat a siliconized coverslip to hold an egg extract in a study. Pluronic F-127 was added to phosphate buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device.

    Get Price
  • Thermoregulation of Shigella and Escherichia coli EIEC ...

    1998-12-1 · The fragment encompassing the intrinsic curvature of the hns promoter region was obtained by PCR amplification of E.coli chromosomal DNA using QC2 (5′-GAA GAC TGA AAG GTC G-3′) and HC9 (5′-CGC ACG AAG AGT ACG G-3′) corresponding respectively to positions 593–608 and 894–909 of the hns sequence (Pon et al., 1988).

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  • Suppressor of Cytokine Signaling 3 Sensitizes Anaplastic ...

    2021-5-12 · Mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) is a maternally inherited mitochondrial disease. Most cases of MELAS are caused by the m.3243A > G variant in the MT-TL1 gene encoding tRNALeu(UUR). However, the genetic cause in 10% of patients with MELAS is unknown. We investigated the pathogenicity of the novel mtDNA variant m.9396G …

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  • Frontiers | Identification of a Novel Variant in MT-CO3 ...

    Human gastric carcinoma shows a higher prevalence of microsatellite instability (MSI) than does any other type of sporadic human cancer. The reasons for this high frequency of MSI are not yet known. In contrast to endometrial and colorectal carcinoma, mutations of the DNA mismatch repair (MMR) genes hMLH1 or hMSH2 have not been described in gastric carcinoma.

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  • Xuemei Yang, Ph.D., Sr. Scientist II - University of ...

    2020-1-29 · Angiotensin 1-7 (Ang 1-7) enhances insulin signaling and glucose transport activity in the skeletal muscle. The aim of our study was to evaluate the effect of AVE0991, a nonpeptide Mas receptor agonist, on the metabolic parameters, expression of RAS components and markers of oxidative stress, and insulin signaling in the skeletal morbidly obese rats. 33-week-old male …

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  • Hypermethylation of the hMLH1 Gene Promoter in Human ...

    Introduction. Plant fruits protect developing seeds and aid in their dissemination (Forlani et al., 2019).They are also an important food source for humans and animals and are rich in nutrients such as carbohydrates, fats, proteins, vitamins, and trace elements (Giovannoni, 2004; Klee and Giovannoni, 2011).Fleshy fruit ripening and the generation of quality attributes occur towards the …

    Get Price
  • Anti-proliferative activity of A. Oxyphylla and its ...

    2020-9-14 · 12% sodium dodecyl sulfate-polyacrylamide gel electro-phoresis and transferred to a nitrocellulose membrane (GVS filter technology, Zola Predosa BO, Italy). The blotted membrane was then blocked with 5% skim milk for 1 hr at room temperature and incubated overnight with specific antibodies at 4°C. After incubation with

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  • A Review on the Modification of Polysaccharide Through ...

    2021-7-13 · BACKGROUND The cytoskeleton actin network of intestinal microvilli has been found to be rapidly impaired after gluten challenge in coeliac disease (CD). The aim of this study was to investigate the presence of an immune reaction towards cytoskeleton structures such as actin filaments in CD. METHODS Eighty three antiendomysial antibody positive CD patients (52 …

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  • Immune reaction against the cytoskeleton in coeliac ... - Gut

    2008-11-24 · Protein samples (30 μg) were separated by SDS-polyacrylamide gel electrophoresis using a 10% polyacrylamide gel, as previously described. 16 Proteins separated in the gel were electroblotted onto nitrocellulose membrane (Hybond ECL; Amersham Biosciences, Amersham, UK) in blotting solution containing 48 mmol/L Tris, 39 mmol/L glycine, 0.037% ...

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  • An abnormal gene expression of the β‐adrenergic system ...

    2011-1-17 · Italy 800 012575 Netherlands 020 547 2600 Spain 901 11 68 90 Sweden 08 506 4 8960 Switzerland 0848 8035 60 UK/Ireland 0845 712 5292 All Other Countries Please contact your local distributor. A complete list of distributors is available at www.genomics.agilent.com.

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  • Manual: QuikChange II Site-Directed Mutagenesis Kit

    2021-5-5 · After the first report of the disease in Italy in 1952, the causative agent of the disease was detected from infected sugar beet plants in Japan a few years later (Tamada & Baba, 1973). Infection with BNYVV results in a reduced taproot weight, massive proliferation of lateral rootlets, and a brownish discolouration of the vascular system.

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  • The Beta vulgaris‐derived resistance gene Rz2 confers ...

    2021-5-12 · Mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes (MELAS) is a maternally inherited mitochondrial disease. Most cases of MELAS are caused by the m.3243A > G variant in the MT-TL1 gene encoding tRNALeu(UUR). However, the genetic cause in 10% of patients with MELAS is unknown. We investigated the pathogenicity of the novel mtDNA variant m.9396G …

    Get Price
  • Frontiers | Identification of a Novel Variant in MT-CO3 ...

    2016-1-8 · Several in vitro studies document the function of the transcriptional regulator TrmBL1 of Pyrococcus furiosus. These data indicate that the protein can act as repressor or activator and is mainly involved in transcriptional control of sugar uptake and in the switch between glycolysis and gluconeogenesis. The aim of this study was to complement the in vitro data with an in vivo …

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  • Genome-wide binding analysis of the transcriptional ...

    2021-4-15 · A novel suicide gene therapy approach was tested in U87 MG glioblastoma multiforme cells. A 26nt G-rich double-stranded DNA aptamer (AS1411) was integrated into a vector at the 5′ of a mammalian codon-optimized saporin gene, under CMV promoter. With this plasmid termed “APTSAP”, the gene encoding ribosome-inactivating protein saporin is driven intracellularly by …

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  • Frontiers | Aptamer-Driven Toxin Gene Delivery in U87 ...

    2010-2-16 · The bioremediation of soils impacted by diesel fuels is very often limited by the lack of indigenous microflora with the required broad substrate specificity. In such cases, the soil inoculation with cultures with the desired catabolic capabilities (bioaugmentation) is an essential option. The use of consortia of microorganisms obtained from rich sources of microbes (e.g., …

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