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polyacrylamide tga in nepal

  • Polyacrylamide Hydrogel: An Alternative Osteoarthritis ...

    Lin, D. C., Yurke, B., Langrana, N. A. Mechanical properties of a reversible, DNA-crosslinked polyacrylamide hydrogel. J Biomech Eng. 126, (1), 104-110 (2004). Lin, D. C., Yurke, B., Langrana, N. …

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  • Preparation of DNA-crosslinked Polyacrylamide

    2009-12-23 · At position 1,838, T was inserted into exon 8, and an internal termination codon (TGA at position 1,899) occurred in the wx-ca allele (Fig. 3b). The three other polymorphic sites in the coding sequence were an A-to-G base substitution in exon 7 (position 1,674), a C-to-G base substitution in exon 8 (position 2,027), and an A-to-C base substitution in exon 10 (position 2,541).

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  • Waxy strains of three amaranth grains raised by

    Lin, D. C., Yurke, B., Langrana, N. A. Mechanical properties of a reversible, DNA-crosslinked polyacrylamide hydrogel. J Biomech Eng. 126, (1), 104-110 (2004). Lin, D. C., Yurke, B., Langrana, N. A. Use of rigid spherical inclusions in young's moduli determination: Application to DNA-crosslinked gels. J Biomech Eng. 127, (4), 571-579 (2005).

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  • Preparation of DNA-crosslinked Polyacrylamide

    G2P[4] was identified as the dominant genotype circulating during this period and polyacrylamide gel electrophoresis revealed the majority of samples exhibited a conserved electropherotype.

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  • Pathogens | Free Full-Text | Characterisation of a

    A total of 38 G2P[4] samples were analysed using polyacrylamide gel electrophoresis to visualise the electropherotype pattern. The majority of samples had a highly similar electropherotype, indicating that a relatively conserved strain was circulating during the outbreak (data not shown).

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  • Pathogens | Free Full-Text | Characterisation of a

    2008-3-1 · Therefore, discrimination of yak CSN3 from the bovine CSN3 variants by PCR-RFLP requires at least 2 enzymes including TaqI and one chosen of the group HindIII, HinfI, and PstI. Reliable discrimination of the yak variants AY095311 and AY095312 by HinfI and PstI furthermore will require high-resolution agarose or even polyacrylamide gel electrophoresis to make the 12-bp difference clearly …

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  • Genetic Variation in the κ-Casein Gene (CSN3) of

    2015-11-11 · The products amplified by PCR with 100pb size were visualized by electrophoresis in 6% polyacrylamide gels revealed with silver stain . Statistical analysis The obtained results were inserted in a specific data bank generated by the statistical software Epi …

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  • Prevalence of toxoplasmosis in pregnant women and

    2008-2-12 · Following purification, the purified protein was shown to produce one predominant band of 61 kDa on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). The protein concentration was determined using a protein assay kit (Bio-Rad Laboratories, Hercules, CA) with bovine serum albumin as a standard.

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  • Preparation of DNA-crosslinked Polyacrylamide

    O nosso laboratório desenvolveu hidrogéis de poliacrilamida reticulada-ADN, um sistema dinâmico de hidrogel, para compreender melhor...

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  • Pathogens | Free Full-Text | Characterisation of a

    In May, 2017, an outbreak of rotavirus gastroenteritis was reported that predominantly impacted Aboriginal children ≤4 years of age in the Kimberley region of Western Australia. G2P[4] was identified as the dominant genotype circulating during this period and polyacrylamide gel electrophoresis revealed the majority of samples exhibited a conserved electropherotype.

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  • American Journal of Botany - Wiley Online Library

    Grasspea (Lathyrus sativus L.) is an excellent candidate crop to provide protein and starch for human diets and animal feed in many countries, such as Bangladesh, China, India, Nepal, and Pakistan, and to a lesser extent in many countries of Europe, the Middle East, North Africa, and in Chile and Brazil in South America ().This crop has an amazing capability to tolerate harsh growth conditions ().

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  • Development of a set of SSR markers for genetic ...

    2017-10-1 · Corchorus capsularis (white jute) and C. olitorius (dark jute) are the two principal cultivated species of jute that produce natural bast fiber of commercial importance. We have identified 4509 simple sequence repeat (SSR) loci from 34,163 unigene sequences of C. capsularis to develop a non-redundant set of 2079 flanking primer pairs. Among the SSRs, trinucleotide repeats were most frequent ...

    Get Price
  • TMBIM6 (transmembrane BAX inhibitor motif

    2015-1-1 · Protein extracts (30 µg) were separated on polyacrylamide gels and transferred to PVDF membranes for 40 min at 15 V using a semi-dry transfer system (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% nonfat dry milk in 0.05% Tween-Tris-buffered saline (1x TBST; 20 mM Trizma base, pH 7.6, 136.9 mM NaCl, 500 µl Tween 20) for 60 min at ...

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  • Brilliant Green and Acid Orange 74 Dyes Removal

    The purpose of this study was to use low cost and easily accessible biosorbent for batch-scale elimination of brilliant green and acid orange 74 dyes from aqueous solution. Pinus roxburghii leaves were utilized to study their dye-eliminating capacities. The adsorbent was characterized by FTIR, TGA, DTA, and SEM. The optimized conditions for brilliant green and acid orange 74 dye ...

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  • Molecular cloning of the potato Gro1‐4 gene

    TaqI RFLPs of the Gro1 candidate gene family.. Genomic DNA of different potato genotypes was restricted with TaqI, separated on denaturing 4% polyacrylamide gels, blotted, and hybridized to the NB domain homologous marker St332 (Accession U60080).Diploid clones P40 and P18 were the resistant (Gro1/gro1) and susceptible (gro1/gro1) parents, respectively, of the F1840 mapping population.

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  • Binod Pandey - Senior Scientist - Novartis Gene

    Polyacrylamide gel elctrophoresis (SDS PAGE gel for proteins as well as PAGE gel for DNA analysis). Atomic Force Microscopy, TGA, QCM. Scanning electron microscopy.

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  • Diversity and phylogeny of soybean rhizobia in central ...

    2014-1-21 · Soybean is the most important crop legume with the highest share of biological nitrogen fixation among cultivated legumes. During the early years following the introduction of soybean cultivation in India, the effects of rhizobial inoculation were impressive, but they have declined over time due to naturalization of the introduced strains. We have characterized the diversity of soybean ...

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  • Production of monoclonal antibodies against hepatitis

    2008-2-12 · Nine murine monoclonal antibodies (mAbs) generated against a recombinant ORF2 protein (amino acids 111–660) of a genotype 4 hepatitis E virus (HEV) strain recognized four sets of epitopes by pairwise competitive ELISA. One mAb (H6225) was able to capture HEV efficiently regardless of genotype and was tested for its ability to neutralize a genotype 3 HEV strain (JE03-1760F) in a …

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  • TMBIM6 (transmembrane BAX inhibitor motif

    2015-8-25 · Protein extracts (30 µg) were separated on polyacrylamide gels and transferred to PVDF membranes for 40 min at 15 V using a semi-dry transfer system (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% nonfat dry milk in 0.05% Tween-Tris-buffered saline (1x TBST; 20 mM Trizma base, pH 7.6, 136.9 mM NaCl, 500 µl Tween 20) for 60 min at ...

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  • Polymer chemistry: Plastic testing lab | EAG

    TaqI RFLPs of the Gro1 candidate gene family.. Genomic DNA of different potato genotypes was restricted with TaqI, separated on denaturing 4% polyacrylamide gels, blotted, and hybridized to the NB domain homologous marker St332 (Accession U60080).Diploid clones P40 and P18 were the resistant (Gro1/gro1) and susceptible (gro1/gro1) parents, respectively, of the F1840 mapping population.

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  • Molecular cloning of the potato Gro1‐4 gene

    2013-9-22 · Sand fly salivary proteins are on the spotlight to become vaccine candidates against leishmaniasis and to markers of exposure to sand fly bites due to the host immune responses they elicit. Working with the whole salivary homogenate entails serious drawbacks such as the need for maintaining sand fly colonies and the laborious task of glands dissection. In order to overcome these difficulties ...

    Get Price
  • Molecular and Immunogenic Properties of Apyrase

    2000-2-12 · Fabrício R. Santos, Arpita Pandya, Manfred Kayser, R. John Mitchell, Aiping Liu, Lalji Singh, Giovanni Destro-Bisol, Andrea Novelletto, Raheel Qamar, S. Qasim Mehdi, Raju Adhikari, Peter de Knijff, Chris Tyler-Smith, A polymorphic L1 retroposon insertion in the centromere of the human Y chromosome, Human Molecular Genetics, Volume 9, Issue 3, 12 February 2000, Pages 421–430, https://doi.org ...

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  • polymorphic L1 retroposon insertion in the

    2007-10-8 · About 20 μg of total protein was separated by electrophoresis on 10% SDS‐polyacrylamide gels embedded with 0.1 mg ml −1 myelin basic protein (MBP) in separating gel as a …

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  • Overexpression of Arabidopsis MAP kinase kinase 7

    The present study investigated the effects of dual specificity phosphatase 1 (DUSP1) gene silencing using lentiviral vector-mediated small interfering (si)RNA on the release of proinflammatory cytokines through the regulation of the mitogen‑activated protein kinase (MAPK) signaling pathway in mice with acute pancreatitis (AP). Two siRNA‑DUSP1 sequences and one scramble siRNA sequence were ...

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  • Effects of silencing the DUSP1 gene using lentiviral ...

    2015-6-6 · The present study was proposed to elucidate the prophylactic role of curcumin in the prevention of hypoxia-induced cerebral edema (HACE). Rats were exposed to simulated hypobaric hypoxia at 7620 m for 24 h at 25 ± 1 °C. Transvascular leakage, expression of transcriptional factors (nuclear factor-kappa B (NF-κB) and hypoxia inducible factor 1 alpha (Hif-1α) and also the genes …

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  • Krishna Kharel - Analytical Research & Development ...

    Jamie Potter | Bay of Plenty, New Zealand | Reservoir Engineer at Mercury NZ | I enjoy working in teams to find ethical, environmental, and efficient solutions to energy problems. | 500+ connections | See Jamie's complete profile on Linkedin and connect

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  • Contribution of IL-1β and TNF-α to the initiation of

    2019-10-29 · The protected RNA was then phenolized, precipitated, and analyzed on a 5% denaturing polyacrylamide gel. After electrophoresis, the gel was dried under vacuum and subjected to autoradiography, and the quantity of protected labeled RNA was determined using densitometry and the NIH Image 1.63 software (National Institutes of Health, Bethesda, MD).

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  • gel electrophoresis 3d obj - TurboSquid

    2021-1-19 · M.Sc. Chemistry-Graduate, Tribhuvan University, Nepal, 2012 A Dissertation Submitted to the Graduate School of the University of Missouri-St. Louis In partial fulfillment of the requirements for the degree Doctor of Philosophy in chemistry, December 2020 Advisory Committee Chair and Advisor Keith J. Stine, Ph.D. Alexei V. Demchenko, Ph.D.

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  • Applications of Nanoporous Gold to Drug Release and ...

    2020-5-19 · R 5’ ACC TGA GTG TCC AGA CCT ATT 3’ 94°C 5min (x1) 94°C 30sec 57°C 30sec 72°C 1min (x35) 72°C 5min (x1) R501X was screened by restriction digest of a 312bp product R501X introduces a NlaIII restriction site. To determine presence of the R501X mutation digest PCR product with NlaIII: Run digests on a 3% (w/v) agarose gel.

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  • Changes in nano-mechanical properties of... |

    Seven micro-liters of the PCR products were loaded to a 6% polyacrylamide gel under standard sequencing conditions in an ALFexpress TM automated DNA sequencer (Amersham-Pharmacia) with the 'fragment' option. A DNA ladder ranging from 50-500 bp, labeled with Cy5, was used to determine the size of the AFLP fragments.

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  • (PDF) Nucleotide diversity in starch synthase IIa and ...

    2007-10-8 · About 20 μg of total protein was separated by electrophoresis on 10% SDS‐polyacrylamide gels embedded with 0.1 mg ml −1 myelin basic protein (MBP) in separating gel as a …

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  • Overexpression of Arabidopsis MAP kinase kinase 7

    Molecular cloning and induction by a two-dimensional polyacrylamide gel electrophoresis: Induction fungus (Fusarium moniliforme) in germinating maize seeds. of pathogenesis related and cellular protectant proteins by jas- Plant Mol. Biol. 16, 517–536. monic …

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  • (PDF) A Novel Rice ( Oryza sativa L.) Acidic PR1 Gene ...

    2019-5-26 · The formation of ordered phases of acid-hydrolyzed cellulose suspensions was studied as a function of cellulose crystallite concentration and added electrolyte (HCl, NaCl, and KCl) concentrations. A chiral nematic phase formed when the suspension concentration was higher than 5.14 × 10-6 nm-3 in water. For biphasic samples, the cellulose concentrations in both isotropic and anisotropic phases ...

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  • Krishna Kharel - Analytical Research & Development ...

    The term of alum refers to various isomorphous solid sulfates composed of trivalent metals and univalent metals, especially aluminum potassium sulfate, AlK (SO 4) 2 ·12H 2 O, a white, crystalline compound. Alums have the general formula M 2 SO 4 ·M III 2 (SO 4) 3 ·24H 2 O, where M is one of alkali metals (potassium, sodium, rubidium, caesium ...

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  • Preparation of DNA-crosslinked Polyacrylamide

    TCA TTC TGA C GC AAA GGT GC G KTHY CAC TTG 56 Bir 10 bp Toehold dizisi dahildir. R2 30 CAA GTG TAG CGK ACC TTT JGK TCA GAA TGA R2, L2 tamamlayıcıdır Tasarım 2 SA1 14 CGT GGC ATA GGA CT 5 'akridit 46,9 SA2 14 GTT TTK CAA

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  • Preparation of DNA-crosslinked Polyacrylamide

    Previtera, M. L., Langrana, N. A. Preparation of DNA-crosslinked Polyacrylamide Hydrogels. J. Vis. Exp. (90), e51323, doi:10.3791/51323 (2014).

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  • Pathogens | Free Full-Text | Characterisation of a

    In May, 2017, an outbreak of rotavirus gastroenteritis was reported that predominantly impacted Aboriginal children ≤4 years of age in the Kimberley region of Western Australia. G2P[4] was identified as the dominant genotype circulating during this period and polyacrylamide gel electrophoresis revealed the majority of samples exhibited a conserved electropherotype.

    Get Price
  • Development of a set of SSR markers for genetic ...

    2017-10-1 · Corchorus capsularis (white jute) and C. olitorius (dark jute) are the two principal cultivated species of jute that produce natural bast fiber of commercial importance. We have identified 4509 simple sequence repeat (SSR) loci from 34,163 unigene sequences of C. capsularis to develop a non-redundant set of 2079 flanking primer pairs. Among the SSRs, trinucleotide repeats were most frequent ...

    Get Price
  • TMBIM6 (transmembrane BAX inhibitor motif

    2015-1-1 · Protein extracts (30 µg) were separated on polyacrylamide gels and transferred to PVDF membranes for 40 min at 15 V using a semi-dry transfer system (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% nonfat dry milk in 0.05% Tween-Tris-buffered saline (1x TBST; 20 mM Trizma base, pH 7.6, 136.9 mM NaCl, 500 µl Tween 20) for 60 min at ...

    Get Price
  • MDPI | Article Reprints Order

    Pyrolysis of Low Density Polyethylene: Kinetic Study Using TGA Data and ANN Prediction. Next Article in Special Issue. One-Step Preparation of Antifouling Polysulfone Ultrafiltration Membranes via Modification by a Cationic Polyelectrolyte Based on Polyacrylamide. Previous Article in Journal.

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  • Diverse endophytic nitrogen-fixing bacteria isolated

    2007-12-4 · Twenty-three nitrogen-fixing bacteria were isolated from surface-sterilized stems and roots of wild rice Oryza rufipogon. Four clusters were defined among these bacteria by SDS-PAGE protein patterns and further confirmed by IS-PCR finger-printing analysis. Phylogenetic analysis of 16S rRNA gene sequences showed that the representative strains LS 8 and LS 18 of cluster II formed a …

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  • Diversity and phylogeny of soybean rhizobia in central ...

    2014-1-21 · Soybean is the most important crop legume with the highest share of biological nitrogen fixation among cultivated legumes. During the early years following the introduction of soybean cultivation in India, the effects of rhizobial inoculation were impressive, but they have declined over time due to naturalization of the introduced strains. We have characterized the diversity of soybean ...

    Get Price
  • Highly adaptable and sensitive FRET-based aptamer

    2020-12-15 · The CdTe QDs were prepared as described in our previous reports (34) wherein 1 mM of CdCl 2.5H2O along with 0.3 mM of TGA, 0.24 mM of Na 2 TeO 3 and 0.1 g of trisodium citrate, respectively was dissolved in 15 mL of ultra-pure water. The pH was adjusted to 10.5 and the mixture was subjected to vigorous shaking at ambient atmospheric condition.

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  • Binod Pandey - Senior Scientist - Novartis Gene

    Polyacrylamide gel elctrophoresis (SDS PAGE gel for proteins as well as PAGE gel for DNA analysis). Atomic Force Microscopy, TGA, QCM. Scanning electron microscopy.

    Get Price
  • Applications of Nanoporous Gold to Drug Release and ...

    2021-1-19 · M.Sc. Chemistry-Graduate, Tribhuvan University, Nepal, 2012 A Dissertation Submitted to the Graduate School of the University of Missouri-St. Louis In partial fulfillment of the requirements for the degree Doctor of Philosophy in chemistry, December 2020 Advisory Committee Chair and Advisor Keith J. Stine, Ph.D. Alexei V. Demchenko, Ph.D.

    Get Price
  • polymorphic L1 retroposon insertion in the

    2000-2-12 · Fabrício R. Santos, Arpita Pandya, Manfred Kayser, R. John Mitchell, Aiping Liu, Lalji Singh, Giovanni Destro-Bisol, Andrea Novelletto, Raheel Qamar, S. Qasim Mehdi, Raju Adhikari, Peter de Knijff, Chris Tyler-Smith, A polymorphic L1 retroposon insertion in the centromere of the human Y chromosome, Human Molecular Genetics, Volume 9, Issue 3, 12 February 2000, Pages 421–430, https://doi.org ...

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  • Molecular Vision: Nintedanib inhibits TGF-β

    2018-12-15 · Introduction. Glaucoma is a leading cause of irreversible blindness [].In 2010, the World Health Organization estimated that 285 million people in the world were visually impaired; in 22.8 million (8%) of these people, the impairment was caused by glaucoma [].The vision loss caused by glaucoma is mainly attributed to damage at the optic nerve head (ONH) as a result of elevated intraocular ...

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  • Review: Efficiently performing periodic elements

    2020-8-9 · Arsenic (As) toxicity is a global phenomenon, and it is continuously threatening human life. Arsenic remains in the Earth’s crust in the forms of rocks and minerals, which can be released into water. In addition, anthropogenic activity also contributes to increase of As concentration in water. Arsenic-contaminated water is used as a raw water for drinking water treatment plants in many parts ...

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  • Expression and Production of Therapeutic

    2011-5-19 · Recombinant human platelet-derived growth factor-BB (rhPDGF-BB) is widely used in many therapeutic applications. Until now, there has been no report on rhPDGF-BB expressed in fungi. In this study, we tested whether Pleurotus eryngii could support the expression of human therapeutic rhPDGF-BB protein. A binary vector pCAMBIA1304 containing the hPDGF-BB gene was constructed and …

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  • A review of the interfacial characteristics of polymer ...

    A review of the interfacial characteristics of polymer nanocomposites containing carbon nanotubes. Junjie Chen *, Baofang Liu, Xuhui Gao and Deguang Xu Department of Energy and Power Engineering, School of Mechanical and Power Engineering, Henan Polytechnic University, 2000 Century Avenue, Jiaozuo, Henan 454000, China. E-mail: [email protected]; [email protected]; Tel: +8615138057627

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  • Molecular and Immunogenic Properties of Apyrase

    2013-9-22 · Sand fly salivary proteins are on the spotlight to become vaccine candidates against leishmaniasis and to markers of exposure to sand fly bites due to the host immune responses they elicit. Working with the whole salivary homogenate entails serious drawbacks such as the need for maintaining sand fly colonies and the laborious task of glands dissection. In order to overcome these difficulties ...

    Get Price
  • Near-Universal Prevalence of Pneumocystis and

    2012-10-16 · Sergio L. Vargas, Carolina A. Ponce, Miriam Gallo, Francisco Pérez, J.-Felipe Astorga, Rebeca Bustamante, Magali Chabé, Isabelle Durand-Joly, Pablo Iturra, Robert F. Miller, El Moukthar Aliouat, Eduardo Dei-Cas, Near-Universal Prevalence of Pneumocystis and Associated Increase in Mucus in the Lungs of Infants With Sudden Unexpected Death, Clinical Infectious Diseases, Volume …

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  • Polymer chemistry: Plastic testing lab | EAG

    2020-10-19 · Benefits of using the method. A schematic of a Dynamic Vapor Sorption (DVS) automated gravimetric sorption system is shown in Figure 1. The Surface Measurement Systems DVS instrument, now in use by many companies and research laboratories world-wide, from pharmaceutical, food, personal care and health to energy, aerospace, agriculture and environment and building materials …

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  • Frontiers | Elicitation of Highly Pathogenic Avian ...

    2021-2-5 · The study was aimed to evaluate the elicitation of highly pathogenic avian influenza (HPAI) virus (AIV) M2e and HA2-specific immunity in chicken to develop broad protective influenza vaccine against HPAI H5N1. Based on the analysis of Indian AIV H5N1 sequences, the conserved regions of extracellular domain of M2 protein (M2e) and HA2 were identified.

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  • Journal of Applied Glycoscience - JST

    Most glucoamylases have either Ser or Gly at this position, whereas only Thermoactinomyces vulgaris R-47 glucoamylase (TGA) and two putative glucoamylases have Trp. We focused on Trp622 in TGA and examined the pH optima of five mutants, W622C, W622D, W622G, W622H and W622S.

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  • Molecular and Immunogenic Properties of Apyrase

    2013-9-22 · Sand fly salivary proteins are on the spotlight to become vaccine candidates against leishmaniasis and to markers of exposure to sand fly bites due to the host immune responses they elicit. Working with the whole salivary homogenate entails serious drawbacks such as the need for maintaining sand fly colonies and the laborious task of glands dissection.

    Get Price
  • Reoviridae - dsRNA Viruses - dsRNA Viruses (2011) -

    The genome segments of HeIRV-2 range in estimated size from about 3.9 to 1.35 kbp, with a 4-6 electrophoretic migration pattern by 12.5% polyacrylamide gel electrophoresis (PAGE). DoIRV-4 contains an estimated 23.4 kbp of dsRNA, with the estimated lengths of individual segments ranging from about 3.8 to 0.7 kbp and a 5-3-2 electrophoretic ...

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  • pcbn inserts in solid cbn and tipped cbn for hard

    2020-5-8 · PCBN inserts for turning cast iron and hardened steel: pcbn is the second hardest material in the world, and cbn related high precision cutting tools are introduced to industry, achieved high productivity and cost reductions. pcbn is the short name of polycrystalline cubic boron nitride, and pcbn inserts are mainly for the hard metal turning to replace the conventional machining way of grinding.

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  • Production of monoclonal antibodies against hepatitis

    2008-2-12 · Nine murine monoclonal antibodies (mAbs) generated against a recombinant ORF2 protein (amino acids 111–660) of a genotype 4 hepatitis E virus (HEV) strain recognized four sets of epitopes by pairwise competitive ELISA. One mAb (H6225) was able to capture HEV efficiently regardless of genotype and was tested for its ability to neutralize a genotype 3 HEV strain (JE03-1760F) in a …

    Get Price
  • Overexpression of Arabidopsis MAP kinase kinase 7

    2007-10-8 · About 20 μg of total protein was separated by electrophoresis on 10% SDS‐polyacrylamide gels embedded with 0.1 mg ml −1 myelin basic protein (MBP) in separating gel as a …

    Get Price
  • (PDF) An outbreak of Salmonella Typhimurium phage

    Salmonella is an important foodborne pathogen, with eggs and egg-containing foods being frequently implicated in causing outbreaks of disease. In April 2012, an investigation was commenced after a number of cases of salmonellosis were linked to a

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  • Research at the IDM | Institute Of Infectious Disease

    2021-7-15 · Research at the IDM. Research in the IDM is led by over 34 independent principal investigators in the basic, clinical and public health sciences, and has a strong translational focus. Grant and contract funding is sourced from the US National Institutes of Health, the Bill & Melinda Gates Foundation, The Wellcome Trust, EDCTP, the South African ...

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  • US6258537B1 - Method of diagnosing gummy stem

    The present invention provides a sensitive test for objectively diagnosing the presence of Didymella bryoniae , the causative agent of gummy stem blight, and differentiating it from similar, nonpathogenic Phoma species. The assay is applicable to DNA isolated from extracts from plant leaves, stem or seed. The detection method employs a polymerase chain reaction technique, using specific ...

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