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    2014-7-1 · 4. After polymerization is complete (30 minutes), pour off the overlay and wash the top of the gel several times with deionized H2O to remove any unpolymerized acrylamide. Drain as much fluid as possible from the top of the gel, and then remove any …

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    2021-3-4 · 13. Apply 5-20 µg total proteins of cell or tissue lysate to each well of a 0.75–1.0 mm thick gel. For th icker gels (1.5 mm thick), apply up to 25-40 µg in each well. 14. Use special gel loading tips or a micro-syringe to load the complete sample in a narrow well with limited movement at the bottom of the well, resulting in high quality data.

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