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working principle of nonionic polyacrylamide gradient gel electrophoresis

  • The principle and Procedure of Polyacrylamide

    2018-3-5 · Polyacrylamide Gel Electrophoresis (PAGE) When electrophoresis is performed in acrylamide or agarose gels, the gel serves as a size-selective sieve during separation. As proteins move through a gel in response to an electric field, the gel’s pore structure allows smaller proteins to travel more rapidly than larger proteins (Figure 2.1).

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  • The principle and method of polyacrylamide

    2014-2-18 · Denaturing gradient gel electrophoresis (DGGE) is a molecular fingerprinting method that separates polymerase chain reaction (PCR)-generated DNA ... they migrate through a polyacrylamide gel. Upon reaching a threshold ... Use caution when working with these materials.

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2015-11-17 · Principle. The concentration of polyacrylamide gels can be prepared as required in two electrophoresis systems —called “continuous system” and “discontinuous system”. The biggest feature of “discontinuous system” lies in its greatly improved sample separation resolution. Main features of this electrophoresis are: (1) Use of two gel …

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    As you would expect from the name, DGGE works by using a gradient of denaturing strength along either the vertical or horizontal axis of a polyacrylamide gel. DNA samples from various sources are amplified by PCR with identical primers and then electrophoresed in this gel, similar to standard polyacrylamide gel electrophoresis.

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  • Denaturing Gradient Gel Elecrophoresis (DGGE)

    2020-5-14 · After removing the pipette, gently aspirate just a tiny bit of air to create an air bubble (approximately 0.5 mL) in the pipette and allow the air bubble to travel up the pipette to mix the acrylamide on its way up. Slowly pipette the gradient solution into the gel …

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  • Principle and Protocol of Sodium Dodecyl

    2020-12-11 · In the agrose gel electrophoresis the potential difference is applied across the electrodes in a horizontal electrophoretic tank containing agarose gel and biomolecules (such as nucleic acid or proteins) is loaded, then molecules migrated to their respective electrodes. The rate of migration of charged particles depends on the …

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  • Denaturing Gradient Gel Electrophoresis |

    2020-8-17 · Electrophoresis Principle, affecting factors and types ... Separation is achieved by applying a potential difference across the gel that contains a pH gradient, which is formed by the introduction of the ampholytes (complex mixtures of synthetic polyamino-polycarboxylic acids). ... Two-dimensional polyacrylamide gel electrophoresis:

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  • Polyacrylamide Gel Electrophoresis (PAGE ...

    2014-2-18 · Denaturing gradient gel electrophoresis (DGGE) is a molecular fingerprinting method that separates polymerase chain reaction (PCR)-generated DNA ... they migrate through a polyacrylamide gel. Upon reaching a threshold ... Use caution when working with these materials.

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  • Denaturing Gradient Gel Elecrophoresis (DGGE)

    PAGE (Polyacrylamide Gel Electrophoresis), is an analytical method used to separate components of a protein mixture based on their size. The technique is based upon the principle that a charged molecule will migrate in an electric field towards an electrode with opposite sign.The general electrophoresis techniques cannot be used to determine ...

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  • Polyacrylamide Gel Electrophoresis (Theory) :

    The Introduction of Nonionic Polyacrylamide Sinofloc. Non-ionic polyacrylamide is a water-soluble polymer or polyelectrolyte. Due to its molecular chain contains a certain number of polar groups, it can through the adsorption of solid particles suspended in water, make the bridge between particles or by charge neutralization condensed to

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  • What Are Gradient Gels, Why Use Them, and How

    2018-9-25 · Gel Electrophoresis of Protein From Basic Science to Practical Approach 71 b. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE). In this method, the separation is carried out in a discontinuous buffer system. The ionised molecules migrate between a leading electrol yte (with high mobility) and a terminating

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  • nonionic polyacrylamide method of operation new

    2008-9-9 · Principle of Gel Electrophoresis Agarose Gel Electrophoresis Polyacrylamide Gel Electrophoresis doi: 10.1038/npg.els.0005335 Gel Electrophoresis ENCYCLOPEDIA OF LIFE SCIENCES & 2005, John Wiley & Sons, Ltd. www.els.net 1

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  • Denaturing Gradient Gel Electrophoresis | Cleaver

    Gel shift assays need not be limited to protein–DNA interactions. Protein–RNA and protein–peptide interactions have also been studied using the same electrophoretic principle. Overview of the gel shift assay method. The gel shift assay consists of three key steps: (1) binding reactions, (2) electrophoresis, (3) probe detection.

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  • Gel Electrophoresis of Protein From Basic Science

    2020-8-17 · Electrophoresis Principle, affecting factors and types ... Separation is achieved by applying a potential difference across the gel that contains a pH gradient, which is formed by the introduction of the ampholytes (complex mixtures of synthetic polyamino-polycarboxylic acids). ... Two-dimensional polyacrylamide gel electrophoresis:

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  • Denaturing Gradient Gel Elecrophoresis (DGGE)

    2014-2-18 · Denaturing gradient gel electrophoresis (DGGE) is a molecular fingerprinting method that separates polymerase chain reaction (PCR)-generated DNA ... they migrate through a polyacrylamide gel. Upon reaching a threshold ... Use caution when working with these materials.

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  • nonionic polyacrylamide method of operation

    The Introduction of Nonionic Polyacrylamide Sinofloc. Non-ionic polyacrylamide is a water-soluble polymer or polyelectrolyte. Due to its molecular chain contains a certain number of polar groups, it can through the adsorption of solid particles suspended in water, make the bridge between particles or by charge neutralization condensed to

    Get Price
  • Polyacrylamide Gel Electrophoresis (Theory) :

    PAGE (Polyacrylamide Gel Electrophoresis), is an analytical method used to separate components of a protein mixture based on their size. The technique is based upon the principle that a charged molecule will migrate in an electric field towards an electrode with opposite sign.The general electrophoresis techniques cannot be used to determine ...

    Get Price
  • Gel Electrophoresis - Boston University

    2008-9-9 · Principle of Gel Electrophoresis Agarose Gel Electrophoresis Polyacrylamide Gel Electrophoresis doi: 10.1038/npg.els.0005335 Gel Electrophoresis ENCYCLOPEDIA OF LIFE SCIENCES & 2005, John Wiley & Sons, Ltd. www.els.net 1

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  • Lecture 13 Electrophoresis (Part-I)

    2017-8-4 · The gel electrophoresis is the best example of zone electrophoresis. ... Principle of the technique: In the discontinuous system, negatively charged ... There are additional accessories needed for casting the polyacrylamide gel such as comb (to prepare different well), spacer, gel caster etc.

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  • Gel Shift Assays (EMSA) | Thermo Fisher

    Gel shift assays need not be limited to protein–DNA interactions. Protein–RNA and protein–peptide interactions have also been studied using the same electrophoretic principle. Overview of the gel shift assay method. The gel shift assay consists of three key steps: (1) binding reactions, (2) electrophoresis, (3) probe detection.

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  • Gel Electrophoresis - The Separation

    1971-6-10 · Cytochrome f was purified from spinach grana membranes with a final yield of 42%. Using technology of disc gel electrophoresis with and without nonionic detergent, it was found that the cytochrome was intrinsically hydrophobic and existed as soluble aggregates at pH 8 which could be disaggregated by 0.1% nonionic detergent to a …

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  • The Use of Disc Gel Electrophoresis with

    2012-4-20 · Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb 1.Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose) subunits 2.During gelation, agarose polymers associate non-covalently and form a network of bundles whose pore sizes determine a gel…

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  • Agarose Gel Electrophoresis for the Separation

    2020-8-17 · Electrophoresis Principle, affecting factors and types ... Separation is achieved by applying a potential difference across the gel that contains a pH gradient, which is formed by the introduction of the ampholytes (complex mixtures of synthetic polyamino-polycarboxylic acids). ... Two-dimensional polyacrylamide gel electrophoresis:

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  • Denaturing Gradient Gel Elecrophoresis (DGGE)

    2014-2-18 · Denaturing gradient gel electrophoresis (DGGE) is a molecular fingerprinting method that separates polymerase chain reaction (PCR)-generated DNA ... they migrate through a polyacrylamide gel. Upon reaching a threshold ... Use caution when working with these materials.

    Get Price
  • Gel Electrophoresis - Boston University

    2008-9-9 · Principle of Gel Electrophoresis Agarose Gel Electrophoresis Polyacrylamide Gel Electrophoresis doi: 10.1038/npg.els.0005335 Gel Electrophoresis ENCYCLOPEDIA OF LIFE SCIENCES & 2005, John Wiley & Sons, Ltd. www.els.net 1

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  • Western Blotting Principle - Bosterbio

    Western blotting principle usually involves two major processes, namely, SDS-polyacrylamide gel electrophoresis and protein blotting and testing. SDS-PAGE vs gel electrophoresis Electrophoresis separation describes a phenomenon that charged particles move towards opposite electrode under the influence of electric field.

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  • (PDF) Gel Electrophoresis, Principle, Types and

    10 Gel Electrophoresis, Principle, Types and Applications Fig.2 Role of SDS in PAGE Fig.3 Cross section of polyacrlamide gel Role of Ammonium persulphate and TEMED Ammonium Persulfate (APS) (NH4)2S2O8 is an oxidizing agent which is used in combination with TEMED to catalyze the polymerization of acrylamide and …

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  • Temperature Gradient Gel Electrophoresis - an

    Denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) are forms of gel electrophoresis that use either a chemical gradient or a temperature gradient to separate samples as they move across an acrylamide gel. DGGE was introduced to microbial ecology by Muyzer et al. in 1993. …

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  • Acid-Urea-Triton Polyacrylamide Gels for

    Experimentally, an optimal balance can be determined by gradient gel electrophoresis with a gradient of urea or Triton . The Triton gradient protocol in the discontinuous gel system, developed by Bonner and coworkers , is described in Section 3. It has a distinct advantage over the urea gradient protocol.

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  • Temperature Gradient Gel Electrophoresis - an

    Hartmut Peters, Peter N. Robinson, in Molecular Diagnostics (Second Edition), 2010. 6.1 Introduction. Temperature gradient gel electrophoresis (TGGE) and the related method denaturing-gradient gel electrophoresis (DGGE) are both based on the principle that the electrophoretic mobility of double-stranded DNA fragments is significantly reduced by …

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  • Basics and recent advances of two

    2014-4-15 · Gel- based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional- polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to simultaneously fractionate, identify, and quantify proteins when coupled with …

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  • Electrophoresis Principle, affecting factors and

    2020-8-17 · Electrophoresis Principle, affecting factors and types ... Separation is achieved by applying a potential difference across the gel that contains a pH gradient, which is formed by the introduction of the ampholytes (complex mixtures of synthetic polyamino-polycarboxylic acids). ... Two-dimensional polyacrylamide gel electrophoresis:

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  • How SDS-PAGE Works - Bitesize Bio

    2016-7-13 · How SDS-PAGE Works. SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) is commonly used in the lab for the separation of proteins based on their molecular weight. It’s one of those techniques that is commonly used but not frequently fully understood. So let’s try and fix that. SDS-PAGE separates proteins …

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  • Western Blotting Principle - Bosterbio

    Western blotting principle usually involves two major processes, namely, SDS-polyacrylamide gel electrophoresis and protein blotting and testing. SDS-PAGE vs gel electrophoresis Electrophoresis separation describes a phenomenon that charged particles move towards opposite electrode under the influence of electric field.

    Get Price
  • BASIC PRINCIPLES OF ISOELECTRIC FOCUSING IN

    2014-4-1 · polyacrylamide gel electrophoresis. Introduction . In isoelectric focusing (IEF), proteins are applied to polyacrylamide gels (IEF gels) or immobilized pH gradient (IPG) strips containing a fixed pH gradient. An electrical field is applied and the protein sample containing a mixture of proteins migrates through the pH gradient. Individual

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  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-3-5 · Polyacrylamide Gel Electrophoresis (PAGE) When electrophoresis is performed in acrylamide or agarose gels, the gel serves as a size-selective sieve during separation. As proteins move through a gel in response to an electric field, the gel’s pore structure allows smaller proteins to travel more rapidly than larger proteins (Figure 2.1).

    Get Price
  • Principles of Blue Native-PAGE

    2017-10-16 · BN-PAGE or Blue Native Polyacrylamide Gel Electrophoresis is a common and inexpensive technique to resolve protein complexes by molecular weight while retaining their native structure through gel electrophoresis. ... Lack of resolution between protein complexes may also be an issue and would require troubleshooting the gradient gel …

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  • SDS-PAGE- Explore the Principles, Protocols, and ...

    SDS PAGE or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis is a technique used for the separation of proteins based on their molecular weight. It is a technique widely used in forensics, genetics, biotechnology and molecular biology to separate the protein molecules based on their electrophoretic mobility. Principle of SDS-PAGE

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  • How SDS-PAGE Works - Bitesize Bio

    2016-7-13 · How SDS-PAGE Works. SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) is commonly used in the lab for the separation of proteins based on their molecular weight. It’s one of those techniques that is commonly used but not frequently fully understood. So let’s try and fix that. SDS-PAGE separates proteins …

    Get Price
  • Types of Electrophoresis - Principles and

    2021-5-27 · Polyacrylamide gel electrophoresis. there are two types of gels used – dissociating and non-dissociating. The non-dissociating gel separates proteins in the original form thereby conserving the structure, function, and activity of the protein. On the other hand, the dissociating gel denatures protein into polypeptides to find out the ...

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  • Electrophoretic Mobility Shift Assay (EMSA) for

    INTRODUCTION. The electrophoretic mobility shift assay (EMSA) is a rapid and sensitive method to detect protein-nucleic acid interactions 1 – 6.It is based on the observation that the electrophoretic mobility of a protein-nucleic acid complex is typically less than that of the free nucleic acid (Fig. 1).The current, widely-used assay differs little from that …

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  • Explain Electrophoresis, its principle and factors ...

    2021-7-13 · Q.5. (c) Explain Electrophoresis, its principle and factors governing it Ans.5.(c) Electrophoresis: is a technique used to separate and sometimes purify macromolecules - especially proteins and nucleic acids - that differ in size, charge or conformation. As such, it is one of the most widely-used techniques in biochemistry and molecular biology.

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  • BASIC PRINCIPLES OF ISOELECTRIC FOCUSING IN

    2014-4-1 · polyacrylamide gel electrophoresis. Introduction . In isoelectric focusing (IEF), proteins are applied to polyacrylamide gels (IEF gels) or immobilized pH gradient (IPG) strips containing a fixed pH gradient. An electrical field is applied and the protein sample containing a mixture of proteins migrates through the pH gradient. Individual

    Get Price
  • A Guide to Polyacrylamide Gel Electrophoresis and

    2018-3-5 · Polyacrylamide Gel Electrophoresis (PAGE) When electrophoresis is performed in acrylamide or agarose gels, the gel serves as a size-selective sieve during separation. As proteins move through a gel in response to an electric field, the gel’s pore structure allows smaller proteins to travel more rapidly than larger proteins (Figure 2.1).

    Get Price
  • Principles of Blue Native-PAGE

    2017-10-16 · BN-PAGE or Blue Native Polyacrylamide Gel Electrophoresis is a common and inexpensive technique to resolve protein complexes by molecular weight while retaining their native structure through gel electrophoresis. ... Lack of resolution between protein complexes may also be an issue and would require troubleshooting the gradient gel …

    Get Price
  • SDS-PAGE- Explore the Principles, Protocols, and ...

    SDS PAGE or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis is a technique used for the separation of proteins based on their molecular weight. It is a technique widely used in forensics, genetics, biotechnology and molecular biology to separate the protein molecules based on their electrophoretic mobility. Principle of SDS-PAGE

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  • Isolation and characterization of beta-gliadin

    Apparent molecular weights determined by gel filtration and SDS-polyacrylamide gradient gel electrophoresis are between 29 000 and 35 000. Valine is the N-terminal amino acid of all beta-gliadins with the exception of the slowest component in electrophoresis at pH 3.2 the N-terminal amino acid of which is asparagine.

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  • Electrophoretic Mobility Shift Assay (EMSA) for

    Polyacrylamide Gradient Gel Electrophoresis Follow Browse By: Overview Publication Experts Milestone Relate Topics EN CN WORKS ATTR 1 transferrin 2 2 disordered 2 3 membranes 2 4. Comparison 2 5 molecular sieves 2 6 iron metabolism 2 7 tube 2 8 2 9 ...

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  • PAGE - polyacrylamide gradient gel

    INTRODUCTION. The electrophoretic mobility shift assay (EMSA) is a rapid and sensitive method to detect protein-nucleic acid interactions 1 – 6.It is based on the observation that the electrophoretic mobility of a protein-nucleic acid complex is typically less than that of the free nucleic acid (Fig. 1).The current, widely-used assay differs little from that …

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  • Prijs Voor Nonionic Polyacrylamide Hydrogel

    Polyacrylamide gradient gel electrophoresis (PGGE) for dysbetalipoproteinemia. Patterns A to D are visible from left to right. The D pattern has a large LDL species while the C pattern has a small dense LDL species for which the retardation is set as 1.

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  • Two Dimensional Electrophoresis (2DE)

    2018-1-30 · Working Principle: ... reliably on immobilized pH gradient (IPG) gel strips using the IEF Systems. The second‐dimension electrophoresis separates the proteins on the basis of their molecular mass using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) ...

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  • Gel Electrophoresis - Principles and Basics |

    2015-2-2 · On a gel of 1 mm thickness and 15 cm length, an applied voltage of about 150 volts gives a current of about 20 mA (falling during electrophoresis if constant voltage is employed). The bromophenol blue dye front takes about 3 hours to reach the bottom of the gel. Greater voltage speeds up electrophoresis, but generates more heat in the gel.

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  • Molecular Techniques and Methods Native Gel

    2014-1-8 · • Patricia Barril and Silvia Nates (2012). Introduction to Agarose and Polyacrylamide Gel Electrophoresis Matrices with Respect to Their Detection Sensitivities, Gel Electrophoresis - Principles and Basics, Dr. Sameh Magdeldin (Ed.), ISBN: 978-953-51-0458-2. This presentation was prepared as a course handout.

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  • Önder Bozdoğan Kırıkkale University Faculty of

    Elementary bodies (EB) of Chlamydia trachomatis serotypes C, E, and L2 were extrinsically radioiodinated, and whole-cell lysates of these serotypes were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Autoradiography of the polypeptide profiles identified a major su …

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  • Purification and partial characterization of the

    Separating Protein: SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) | Protocol. Poly-Acrylamide Gel Electrophoresis utilizes a hydrogel made from polyacrylamide. Polyacrylamide is a polymer that forms a very regular matrix through which proteins can move. The more concentrated the gel is, the slower the proteins will . Get Price

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  • how can i dissolve a polyacrylamide gel

    2018-10-26 · I understand the basic working principle of laboratory equipment much better now. 4.1: 2: 5: 20: 13: I have easy access to the supplies and equipment for my work in the laboratory. 3.8: 2: 5: 17: 14: My group was good to perform the assigned step of gel electrophoresis. 3.8: 3: 5: 19: 15: After electrophoresis, I was excited to see the …

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